FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005204148

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005204148
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67059164
Sequences flagged as poor quality0
Sequence length35-51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTG10921651.6286588362479437No Hit
CTTCGTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCTCGCC8922781.330583244372089No Hit
GGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCT8534801.272726871453393No Hit
AGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTTCCAT7061601.053040267546431No Hit
CTCTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAG4895720.7300598021174258No Hit
CAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCAT4351360.6488837230359746No Hit
CTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCT3914790.583781509712826No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGAT3772400.5625480210281179No Hit
GCCCTTCGTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCTCG3767970.561887410347078No Hit
CTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGTG3633790.5418782136920168No Hit
CTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAG3497310.5215260363221945No Hit
CTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCG3439530.5129097642791968No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG3386830.5050510322496714No Hit
CGACTGTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGA3323640.4956280099167356No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT3265460.4869520890537794No Hit
GGGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTC3252960.48508806343007793No Hit
GGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA3052400.4551801451029124No Hit
CCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGGCA2992220.44620598014016394No Hit
CTGAGAGATGGGCGAGCGCCGTTCCGAAGGGACGGGCGATGGCCTCCGTTG2953750.44046925488066035No Hit
GTCGCTTTTTGATCCTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGA2898060.4321646479219454No Hit
CTCGGATCGGCCCCGCCGGGGTCGGCCCACGGCCCTGGCGGAGCGCTG2825660.42136821150946646No Hit
CCTTCGTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCTCGC2773930.4136541278683402No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG2766180.4124984319816454No Hit
GTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGA2554570.38094271500312765No Hit
CTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCC2533930.3778648358932718No Hit
GTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATT2459670.366791032467986No Hit
CTTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGG2435980.36325833110594696No Hit
CTCCGAAGTTTCCCTCAGGATAGCTGGCGCTCTCGCAGACCCGACGCACC2394190.35702652064078816No Hit
GCCGAAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGG2325020.34671174844947367No Hit
GTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGG2225190.33182489420834416No Hit
CCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGACATGAGAGGTGT2197240.32765693291374764No Hit
CAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTTCCATGT2082240.310507897175694No Hit
GTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTT2080510.3102499160293737No Hit
CTGCCCTTTGTACACACCGCCCGTCGCTACTACCGATTGGATGGTTTAGTG2078620.30996807535507004No Hit
GCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGT2015200.3005107549506582No Hit
GGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGG1974960.2945100836628384No Hit
GGCGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGACCGATCCCG1965150.29304719635335746No Hit
GTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATT1936850.2888270423412973No Hit
CTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCG1902940.2837703136293199No Hit
GGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACGATCTCAACCTATT1894470.2825072498666998No Hit
CCTGCCCTTTGTACACACCGCCCGTCGCTACTACCGATTGGATGGTTTAGT1889250.28172883276624205No Hit
GTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTTC1865330.2781618333327269No Hit
GGTGTATGTGCTTGGCTGAGGAGCCAATGGGGCGAAGCTACCATCTGTGGG1823290.2718927423550941No Hit
CTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAA1821240.27158704215280705No Hit
TGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTC1786600.26642145434440545No Hit
TGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGC1768830.2637715555177515No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG1709690.254952477486895No Hit
GTGGGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGT1703250.253992131485564No Hit
CTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG1693530.2525426651605737No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC1652930.24648830993479134No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAG1643520.24508507144526884No Hit
GTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCG1597530.23822694837054634No Hit
GTCCTGAGAGATGGGCGAGCGCCGTTCCGAAGGGACGGGCGATGGCCTCCG1587000.2366566931851402No Hit
GTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGG1586840.23663283365715684No Hit
GTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTC1581330.23581117116222924No Hit
CCGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACT1568700.23392775967204124No Hit
CTCGGGGCCACGCGCGCGTCCCCCGAAGAGGGGGACGGCGGAGCGAGCGC1537950.22934225663773558No Hit
CTCAGGATAGCTGGCGCTCTCGCAGACCCGACGCACCCCCGCCACGCAGTT1520620.22675797151303587No Hit
GTCGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCATCTA1512680.22557394243686069No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA1406950.2098072681013441No Hit
GTGGTAAACTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAAC1390690.2073825435700332No Hit
GTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA1380470.20585851622009482No Hit
GTGGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGG1355300.20210511422420954No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1343600.20036038624042496No Hit
GGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCAGG1332670.1987304822350604No Hit
GTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGTG1332230.19866486853310608No Hit
GCCCTTTGTACACACCGCCCGTCGCTACTACCGATTGGATGGTTTAGTG1320550.19692312299031942No Hit
GTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGG1318980.1966890013719825No Hit
GGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTA1290500.19244200539094106No Hit
GTCGCGGTTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCG1246730.18591493326698794No Hit
GGGTAAACGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCG1222560.18231065332099874No Hit
CATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAAT1207820.18011259430552998No Hit
GTGTAAATCTCGCGCCGGGCCGTACCCATATCCGCAGCAGGTCTCCAAGGT1190400.1775148881963396No Hit
CTTGCGTTGATTAAGTCCCTGCCCTTTGTACACACCGCCCGTCGCTACTA1190060.1774641866993749No Hit
TGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACAT1182280.1763040171511831No Hit
CGTTGATTAAGTCCCTGCCCTTTGTACACACCGCCCGTCGCTACTACCGAT1175010.17521989984843833No Hit
GTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGC1174790.17518709299746116No Hit
CCGAAGTTTCCCTCAGGATAGCTGGCGCTCTCGCAGACCCGACGCACCCC1171500.17469648145330294No Hit
ATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACGATCTCAAC1171480.17469349901230502No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTG1171010.17462341164885384No Hit
CGCGATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCA1153500.17201228455517278No Hit
CCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACGATCTCAACCTAT1153030.17194219719172163No Hit
CTCTGATCGTTTTTTCACTGACCCGGTGAGGCGGGGGGGCGAGCCCCGAGG1112250.16586099999695791No Hit
GGCGGAATCAGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCTGG1106500.16500354821005522No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTGCCTACC1099530.1639641675222793No Hit
CTCCAAGGTGAACAGCCTCTGGCATGTTGGAACAATGTAGGTAAGGGAAGT1097130.1636062746025286No Hit
CTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGT1092130.16286066435304802No Hit
CAGGTGGGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGT1088630.16233873717841157No Hit
TGTAAATCTCGCGCCGGGCCGTACCCATATCCGCAGCAGGTCTCCAAGGTG1075740.16041655395525062No Hit
CTTGAAAATCCGGGGGAGAGGGTGTAAATCTCGCGCCGGGCCGTACCCAT1065180.1588418251083476No Hit
GTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGAT1043030.15553877170314856No Hit
CCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAA1021920.15239080522984152No Hit
GTCAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCAT994460.1482959137396941No Hit
CGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGG978330.14589057507486972No Hit
GGCCACTTTTGGTAAGCAGAACTGGCGCTGCGGGATGAACCGAACGCCGGG976770.14565794467703175No Hit
CTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGGCAA961960.14344944711807026No Hit
GTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGACATGAGAGGTGTAGA958220.14289173065145877No Hit
CTCAGCGTGTGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACC951610.1419060339016454No Hit
GTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAATTCT947100.14123349345661393No Hit
GTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCTCGCCCGTC936520.139655782168713No Hit
CGGGTGGTAAACTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTC932900.13911596034808904No Hit
GTGCTTGGCTGAGGAGCCAATGGGGCGAAGCTACCATCTGTGGGATTATG925200.13796772056388892No Hit
CTGGCATGTTGGAACAATGTAGGTAAGGGAAGTCGGCAAGCCGGATCCGT921980.13748754756322343No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAACG898040.13391756568871035No Hit
CTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTCCGTAGCGGTC889700.13267388779257672No Hit
GCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGG887080.13228318802184888No Hit
CGAAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGG885970.1321176625464642No Hit
CCCGTGCCTTGGAAAGCGTCGCGGTTCCGGCGGCGTCCGGTGAGCTCTCG874150.1303550399166921No Hit
CGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCT859270.12813610381423785No Hit
GTCGGTCCTGAGAGATGGGCGAGCGCCGTTCCGAAGGGACGGGCGATGGC848520.12653304177785454No Hit
CTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTGGAGC838100.12497919001793699No Hit
CTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGT830780.1238876166126974No Hit
CAGGTGTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGC829610.12371314381431896No Hit
CGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACGATCTCAACCTATT829310.1236684071993501No Hit
CTCGCTTGATCTTGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGC826390.12323297081365345No Hit
GGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTC825390.12308384876375733No Hit
GTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTG795640.11864746777934781No Hit
CCTCAGGATAGCTGGCGCTCTCGCAGACCCGACGCACCCCCGCCACGCAGT795370.11860720482587586No Hit
CTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACC794390.11846106521697765No Hit
GTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTAT792380.11816132989668644No Hit
AGCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAA790410.11786755945839111No Hit
CCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCG775770.11568441264791192No Hit
CGGGTGTTGACGCGATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTG766820.11434977030134166No Hit
GGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAA761880.11361310737485483No Hit
GCCACTTTTGGTAAGCAGAACTGGCGCTGCGGGATGAACCGAACGCCGGGT761160.11350573949892963No Hit
GGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTCCGTAGCGGTCCTGA759090.11319705685564467No Hit
CTTTGTACACACCGCCCGTCGCTACTACCGATTGGATGGTTTAGTGAGGC756760.11284960247938672No Hit
CCCGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACT756470.11280635708491683No Hit
CTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGTAAGGTCGGCGCG755160.11261100719955291No Hit
CGCTTTTTGATCCTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATT749830.1118161866736066No Hit
GTTCTCTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCG733240.10934225186583No Hit
GTCCGTAGCGGTCCTGACGTGCAAATCGGTCGTCCGACCTGGGTATAGGGG730920.108996288710071No Hit
CTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGG719910.10735445494071474No Hit
CCCCTCGATGCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTT713980.10647016118483077No Hit
CAGAAAAGGTGTTGGTTGATATAGACAGCAGGACGGTGGCCATGGAAGTCG713820.10644630165684736No Hit
GGTAAACTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACA713290.10636726697040243No Hit
GGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGG712940.10631507425293879No Hit
GGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGT708960.10572156849435226No Hit
GCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGC707330.10547849955302156No Hit
CCTGAGAGATGGGCGAGCGCCGTTCCGAAGGGACGGGCGATGGCCTCCGTT703880.10496402848087995No Hit
CGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCAC698920.1042243831133952No Hit
CTTGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTT696480.10386052531164867No Hit
GTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAA692300.10323719514308291No Hit
CTTGTGGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGT690280.10293596860229273No Hit
CCGACTGTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTG679550.1013358890069074No Hit
GTATGTGCTTGGCTGAGGAGCCAATGGGGCGAAGCTACCATCTGTGGGATT677370.10101080293813386No Hit
GTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGAC677310.1010018556151401No Hit
GGCACGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGCG674460.10057685777293615No Hit
GGGAAGTCGGCAAGCCGGATCCGTAACTTCGGGATAAGGATTGGCTCTA671200.1000907198902748No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)