FastQCFastQC Report
Thu 2 May 2024
EGAF00005204149

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005204149
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67059164
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCG4044840.6031748323018163No Hit
CTCGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAA3063510.4568368910772583No Hit
CGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAA2768020.41277281655345416No Hit
CCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTA2352510.350811113601118No Hit
ACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGA2308790.34429149757965966No Hit
CTGACTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCA2198730.32787912476809283No Hit
CTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGTGCGTCGG2123850.3167128656718715No Hit
CGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACT1992990.29719875422246544No Hit
CTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGG1981250.295448061356685No Hit
CCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGA1938400.2890581815186363No Hit
CTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAAC1860770.2774818367852006No Hit
CTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTC1749820.26093674534922623No Hit
CTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCCGCCAAGCC1747410.2605773612089766No Hit
CTCAGACTTTATTCAAAGACCAGGAAGGGCCGGTGCAAGGAGGGGAGGAG1725440.25730114977275886No Hit
CTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGACTTCC1659570.24747848034610154No Hit
CTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCGTGAG1609270.23997764123632678No Hit
CACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCA1520190.22669384903158055No Hit
CGCAGGGCCGGCGGACCCCGCCCCGGGCCCCTCGCGGGGACACCGGGGGG1495480.22300904317864742No Hit
CCTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCT1494340.22283904404176588No Hit
CGAAACCCCGACCCAGAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGT1398840.20859788827668652No Hit
CAAAAGGTCAGAAGGATCGTGAGGCCCCGCTTTCACGGTCTGTATTCGTA1360680.20290739085265064No Hit
GTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGC1333320.19882741156749284No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCA1293750.19292665205310344No Hit
CAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATGGGAATAACGC1256140.18731817175651042No Hit
CTCCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGC1255390.1872063302190883No Hit
CAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTA1244310.18555405790623933No Hit
CATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCAC1228990.1832695081018308No Hit
CTTGGAGACCTGCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACC1210440.1805032940762578No Hit
CTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGG1202140.17926558106212No Hit
CCCCGACCCAGAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTTCCCC1167140.17404630931575585No Hit
CTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCATCC1162410.17334096201974722No Hit
GTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCAC1160370.17303675303795915No Hit
CCGAGGCCAACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCG1142850.17042413472377915No Hit
CTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCATTGG1141420.1702108901924277No Hit
CGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCC1101140.16420425402261205No Hit
CTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTT1064410.1587270011299276No Hit
ACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCCGCCAAGC1037660.1547379862952064No Hit
CCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAA1027340.15319904674027848No Hit
CTTGTGTCGAGGGCTGACTTTCAATAGATCGCAGCGAGGGAGCTGCTCTG1008970.15045967468368676No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT1007990.1503135350747886No Hit
CTCAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGG1001360.14932485588397731No Hit
GGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTA998100.14883871800131596No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA962440.14352102570202038No Hit
GTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGATGACGAGG947920.14135577353752873No Hit
CTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCT944170.1407965658504183No Hit
CGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCTATATCAACCAACAC932410.13904289054363994No Hit
CTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTA925430.13800201863536504No Hit
CTGTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTC924410.13784991414447098No Hit
GTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCATGGCAACAA917030.13674939341623762No Hit
CGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCTT895730.1335730937534503No Hit
CGTGAGGCCCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGATCA894570.13340011217557082No Hit
CCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTT878420.13099179106974848No Hit
GGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGT877710.13088591441432226No Hit
CAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAAC873850.13031030330172322No Hit
CTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCT869850.12971381510213875No Hit
CAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCATGGC869770.12970188533814708No Hit
CGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCC868320.1294856583657977No Hit
GCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAA866910.12927539627544418No Hit
CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGC862320.12859092606642097No Hit
GTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTAT861260.1284328566935311No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA852000.12705198651149305No Hit
CTTATCCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTG851040.12690882934359277No Hit
CTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCACGGGAGGTT839120.12513129450883104No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA836900.12480024355806166No Hit
GTGGTATTTCACCGGCGGCCCGCAGGGCCGGCGGACCCCGCCCCGGGCCC836800.12478533135307204No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGA836280.12470778788712605No Hit
CAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCCAGTTATCC826290.12321805860866385No Hit
CCTCGCGGCGCCCATCTCCGCCACTCCGGATTCGGGGATCTGAACCCGA822500.12265288603955755No Hit
CCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCGCCCATCTCCG816490.12175666251968188No Hit
CTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCG809520.12071728183190593No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGA800040.11930360479889073No Hit
CACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCAT797920.11898746605311095No Hit
CTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGCCGAG792910.1182403645831314No Hit
CTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCAT786540.11729045712529312No Hit
ACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCATG786200.11723975562832843No Hit
GTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGC781960.11660747813676889No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC768950.1146674002676204No Hit
GAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCCCGCAGGGCCGGC767350.1144288049877866No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA764800.11404854376055151No Hit
CCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAAT760750.11344459945847223No Hit
GTTGAGATCGTTTCGGCCCCAAGACCTCTAATCATTCGCTTTACCGGATA749870.11182215155560245No Hit
CCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACTAGAGTC746320.11129276827847123No Hit
GTCGCGTTACCGCACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCCGG743460.11086627921576833No Hit
CTCTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCA740510.11042636916857478No Hit
GGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTG740330.11039952719959349No Hit
CTCGCGGCGCCCATCTCCGCCACTCCGGATTCGGGGATCTGAACCCGACT728520.10863839579032032No Hit
CTCCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCGCCCATCTC725420.10817611743564234No Hit
CCAAAAGGTCAGAAGGATCGTGAGGCCCCGCTTTCACGGTCTGTATTCGT720100.10738278813019501No Hit
TGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTC714090.10648656461031931No Hit
CTCGTTCATCCATTCATGCGCGTCACTAATTAGATGACGAGGCATTTGG713640.10641945968786608No Hit
CCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCC713460.10639261771888478No Hit
GTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCGACCC711510.10610182972158734No Hit
CCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCG711260.1060645492091133No Hit
CGAGGGCTGACTTTCAATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTA710020.10587963786724212No Hit
CAAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGCAGCGAGGGAG709390.10578569097580758No Hit
TTTCAATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTACGAAACCCCGA705440.1051966588787179No Hit
CATTGTTCCAACATGCCAGAGGCTGTTCACCTTGGAGACCTGCTGCGGAT703530.10491183576341631No Hit
CCCATTCCAGGGCGCCCTGCCCTTCACAAAGAAAAGAGAACTCTCCCCGG696380.10384561310665906No Hit
CTTTTCTGGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTT694540.10357122853485021No Hit
CTCGGGGCTCGCCCCCCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAG688690.1026988645429579No Hit
CTGCCCTTCACAAAGAAAAGAGAACTCTCCCCGGGGCTCCCGCCGGCTT673330.10040834985655354No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT673090.10037256056457847No Hit
CCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGTCC672930.10034870103659509No Hit
GTTACTCCCGCCGTTTACCCGCGCTTCATTGAATTTCTTCACTTTGACAT671740.10017124579721871No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)