FastQCFastQC Report
Fri 3 May 2024
EGAF00005204151

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005204151
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54871966
Total Bases5.5 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCG1861010.33915497031763No Hit
CCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTT1393420.2539402360761049No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA1370570.2497759967266345No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT1278130.23292950720956487No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG1145570.20877145170996791No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT1089430.19854036212225384No Hit
CTCAGACTTTATTCAAAGACCAGGAAGGGCCGGTGCAAGGAGGGGAGGAG1052410.19179374764884496No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC1038120.18918950343423088No Hit
CTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTC1013070.18462433075570867No Hit
CGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAA1003330.18284928956254276No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGG939900.17128965271628868No Hit
CAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAG937750.17089783150835164No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG930380.16955470485602794No Hit
CTCGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAA927980.16911732304251684No Hit
CCTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCT897270.16352065825379758No Hit
CCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTA827020.15071812808748278No Hit
CTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGG784520.1429728251398902No Hit
CACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCA783480.14278329302070206No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA737780.13445481432176132No Hit
CACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCA726920.13247566161562355No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA694490.12656553986055466No Hit
ACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGA694160.12650539986119688No Hit
CTGACTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCA688240.1254265247212028No Hit
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT671190.12231929142105097No Hit
CTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGG670980.12228102051236873No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCT662160.12067364234771541No Hit
CTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACC648100.11811131389022947No Hit
CTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCCGCCAAGCC620520.11308506788329764No Hit
CGCAGGGCCGGCGGACCCCGCCCCGGGCCCCTCGCGGGGACACCGGGGGG606630.11055372063760209No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT594250.10829755944957395No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC589310.10739728188343023No Hit
CTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCG580900.10586462311191838No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGA580850.10585551099080356No Hit
CTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGTGCGTCGG580330.1057607449312095No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC575570.10489327100107913No Hit
CGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCCA568640.1036303310145658No Hit
GGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGA551040.10042286438215098No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)