FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005204241

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005204241
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13299867
Sequences flagged as poor quality0
Sequence length35-51
%GC65

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1681641.2644036214798238No Hit
CACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTCCGGCCG1413221.0625820543919724No Hit
GTCCGTCCTTCCGTTCGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCGCG1219100.9166257076104596No Hit
CCCACCACCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGGAAGACG955880.7187139540568338No Hit
CCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTCCGGCCG936780.704352908190736No Hit
CCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGGAAGACGAACGGA921840.6931197131520187No Hit
CTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTC873210.6565554377348285No Hit
CGTCCCGGCCCGGAGCGGACGAGCCGCCCCGGCGGTGAACGGGGAGGAGG721810.5427197129114149No Hit
GTCCCGGCCCGGAGCGGACGAGCCGCCCCGGCGGTGAACGGGGAGGAGGCG663700.4990275466664441No Hit
CCACCACCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGGAAGACGA631090.4745085044835411No Hit
CACCACCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGGAAGACGAA546170.41065824192076505No Hit
CTTCCGTTCGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCGCGGGCGTGG535190.4024025202658042No Hit
CTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGGG481450.36199610116401915No Hit
CGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCG424570.31922875619733637No Hit
CGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCG397790.29909321649607473No Hit
CGTCCTTCCGTTCGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCGCGGG393250.2956796485258086No Hit
CCACCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGGAAGACGAACGG363940.27364183416270255No Hit
CCCGGCCCGGAGCGGACGAGCCGCCCCGGCGGTGAACGGGGAGGAGGCGGG311620.23430309491064832No Hit
CTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGTGCGTCGGG310320.23332564152709198No Hit
CCCGTCCCGGCCCGGAGCGGACGAGCCGCCCCGGCGGTGAACGGGGAGG307660.23132562152689196No Hit
CCCACCCCCACCACCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGG305400.22962635641394008No Hit
CGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAA284840.21416755520938668No Hit
CCCCCACCCCCACCACCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGG264080.19855837656120923No Hit
CACCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGGAAGACGAACGG263420.19806213099724984No Hit
CCGTCCCGGCCCGGAGCGGACGAGCCGCCCCGGCGGTGAACGGGGAGGAGG256930.19318238295164908No Hit
CACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGGAAGACGAACGGAAGG252390.1897688149813829No Hit
CCCCACCCCCACCACCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGG240580.18088902693538214No Hit
GTCCTTCCGTTCGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCGCGGGCG239930.18040030024360393No Hit
CGGACCCCGTCCCGGCCCGGAGCGGACGAGCCGCCCCGGCGGTGAACGGGG233960.17591153355142575No Hit
GGTTGAGATCGTTTCGGCCCCAAGACCTCTAATCATTCGCTTTACCGGAT229390.17247540896461597No Hit
ACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGA225720.16971598287411443No Hit
GTTGAGATCGTTTCGGCCCCAAGACCTCTAATCATTCGCTTTACCGGATA223300.16789641580626333No Hit
CGAGGGCTGACTTTCAATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTA219830.16528736715938588No Hit
CCCATGTTCAACTGCTGTTCACATGGAAGCCTTCTCCACTTCGGCCTTCA217550.16357306430207158No Hit
GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTC216900.1630843376102934No Hit
CAGAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTG208640.1568737491886197No Hit
CCCCACCACCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGGAAGACG204290.1536030397897964No Hit
CGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCC203520.15302408663184375No Hit
CGACCCGCGCGCCCTCCCGAGGGAGGACGCGGGGCCGGGGGGCGGAGACGG198330.14912179196979938No Hit
GCCGTCCGTCCTTCCGTTCGTCTTCCTCCCTCCCGGCCTCTCCCGCCGAC197700.1486481030223836No Hit
CTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGG186510.14023448505161742No Hit
CGGCGGGAGAGGCCGGGAGGGAGGAAGACGAACGGAAGGACGGAC183950.13830965377322946No Hit
CACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCA183590.13803897437470614No Hit
GGGTAGCCGACGTCGCCGCCGACCCCGTGCGCTCGCTCCGCCGTCCCCCT183590.13803897437470614No Hit
GCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGC179510.1349712745247753No Hit
CGTCCGTCCTTCCGTTCGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCG175850.13221936730645503No Hit
GTCCGTCCTTCCGTTCGTCTTCCTCCCTCCCGGCCTCTCCCGCCG173670.13058025317095276No Hit
GTCCGTCCTTCCGTTCGTCTTCCTCCCTCCCGGCCTCTCCC169470.1274223268548475No Hit
CCCCCACCACCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGGAAGA167140.1256704296366272No Hit
CCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGA165350.12432455151619186No Hit
CGGGAGAGGCCGGGAGGGAGGAAGACGAACGGAAGGACGGAC165330.12430951377182944No Hit
CCGTCCTTCCGTTCGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCGCGGG165030.12408394760639335No Hit
CTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGG160340.12055759655340914No Hit
GTCCGTCCTTCCGTTCGTCTTCCTCCCTCCCGGCCTCTCCCG155850.11718162294404898No Hit
CGCTTCTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTC155300.11676808497408282No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC154320.11603123550032494No Hit
CGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTT152240.11446731008663472No Hit
CTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACG151810.11414399858284296No Hit
GGGAGAGGCCGGGAGGGAGGAAGACGAACGGAAGGACGGAC150660.11327932828200463No Hit
CTGACTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCAT147930.1112266761765362No Hit
GTCCGGCGCCGTCCGTCCTTCCGTTCGTCTTCCTCCCTCCCGGCCTCTCC147860.11117404407126778No Hit
GTCGGCGGGAGAGGCCGGGAGGGAGGAAGACGAACGGAAGGACGGAC147120.11061764752985875No Hit
CCCCGACCCAGAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTTCCCC146280.10998606226663771No Hit
CTGAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCG142350.10703114549942491No Hit
AGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGT138350.10402359662694372No Hit
CAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATGGGAATAACGC137890.10367772850660838No Hit
GCTTCTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCG136320.1024972655741595No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)