FastQCFastQC Report
Thu 12 May 2022
EGAF00005204261

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005204261
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75528641
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTC4386450.58076644064071No Hit
GGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCAT3711260.4913712137359919No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTC3594460.47590688147030213No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGC3404740.45078793354695734No Hit
CCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCC3394560.4494401004779101No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCG3383500.4479757553164501No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT3024560.40045206162255714No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATC2645160.3502194617800683No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT2565600.3396857094251173No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATC2442610.3234018205093879No Hit
GATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGT2428750.32156675505388743No Hit
CACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAG2265920.3000080459543817No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATC2240270.2966119832607606No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCG2220490.2939931091835745No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGAT2081690.2756159746075664No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA1868950.24744917626678864No Hit
CAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGT1805310.23902323358366795No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGC1802480.238648541286477No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA1738100.2301246225256456No Hit
CATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGGA1677200.22206145613026454No Hit
GGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATT1675190.22179533191918546No Hit
CACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAG1668130.22086058717778334No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACC1668070.22085264317148245No Hit
ATCAAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATACGTCCTCTAT1616870.21407375779474172No Hit
GGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGAT1610470.21322639712264913No Hit
ATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTG1603830.2123472604253531No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGC1537400.20355192144924203No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTT1502780.19896822981364115No Hit
GATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGT1474240.19518953081652826No Hit
GGGAAGCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTGTCACTCCACTC1427870.18905013794700742No Hit
CTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGT1406630.18623795971650012No Hit
CAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTG1403040.18576264333949818No Hit
CTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGA1401590.1855706631872272No Hit
TGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCGGA1391630.18425195814128312No Hit
CATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCT1358070.1798086106169976No Hit
GATCAAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATACGTCCTCTA1282230.1697673866527004No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTT1272250.16844603360465601No Hit
CTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTA1264260.1673881567655904No Hit
GGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATC1206180.15969835866635015No Hit
CAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTG1126200.14910899826729307No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGA1121350.14846685775797291No Hit
AGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCC1071740.14189848854820516No Hit
ATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCG1034860.13701557267527162No Hit
ATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCGG1034730.13699836066161974No Hit
GGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACG1005030.13306607754269006No Hit
CCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCAC978020.1294899507062493No Hit
CATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCG975570.12916557044896385No Hit
CGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAG948110.12552986356526658No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTC944250.12501879915991074No Hit
GTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGT943230.12488375105279598No Hit
GGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCA942120.12473678693622993No Hit
CGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTC926710.12269650131795698No Hit
CTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCA913060.1208892398845095No Hit
CGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTC911690.1207078517406397No Hit
CACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCGGATGTG883120.11692518074037635No Hit
CGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAA881620.11672658058285465No Hit
ATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTA859150.11375155022317958No Hit
GGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTC853180.11296112159624319No Hit
GATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCGGAT843640.11169802459440518No Hit
CTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGG818510.10837080995539161No Hit
GTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGT777950.10300066169600483No Hit
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGG777910.10299536569180424No Hit
GGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGG776850.10285502158048893No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG772860.1023267451614812No Hit
GGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGAT772180.10223671309007136No Hit
TGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAA771940.10220493706486787No Hit
ATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCGGATG767400.1016038405881022No Hit
GTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCG762380.10093919206092958No Hit
CTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCA760580.10070087187190352No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)