FastQCFastQC Report
Fri 10 May 2024
EGAF00005204300

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005204300
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33026642
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCACCAAATCTCGTAT24881817.533860087864822TruSeq Adapter, Index 1 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCACCAAATCTCGTTT6860562.0772805179527487TruSeq Adapter, Index 1 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCACCAAATCTCGGAT3482271.0543820955215488TruSeq Adapter, Index 1 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCACCAAATCGCGTAT2537360.7682767142962944TruSeq Adapter, Index 1 (97% over 38bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2036310.6165658621908943No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCACCAAATCTCGGTT1359900.4117584827425083TruSeq Adapter, Index 1 (97% over 38bp)
CTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGCGTCCTCCCTCGGGAGG1266410.3834510332597543No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCACCAAATCGCGTTT1127190.34129718667734976TruSeq Adapter, Index 1 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCACCAAATCGCGGAT778080.23559161721618563TruSeq Adapter, Index 1 (97% over 38bp)
CCCCTCCCGGCGCCCACCCCCGCGGGGAATCCCCCGCGAGGGGGGTCTCC597870.1810265784816997No Hit
CCCCCCTCCCGGCGCCCACCCCCGCGGGGAATCCCCCGCGAGGGGGGTCT587630.17792605133758377No Hit
CCCCGACCCGCGCGCCCTCCCGAGGGAGGACGCGGGGCCGGGGGGCGGAG554190.16780089238258009No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT487560.14762627093605218No Hit
CCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGCGTCCTCCCTCGGGAG458060.13869408824548374No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCACCAAATCGCGGTT421710.12768782245557994TruSeq Adapter, Index 1 (97% over 38bp)
CTCCCCCCTCCCGGCGCCCACCCCCGCGGGGAATCCCCCGCGAGGGGGGT331120.10025845194918696No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)