FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005204300

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005204300
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33026642
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCACCAAATCTCGTATG22895596.932460769096658TruSeq Adapter, Index 1 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCACCAAATCTCGTTTG6206191.8791465387247062TruSeq Adapter, Index 1 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCACCAAATCTCGGATG3261290.9874724775228435TruSeq Adapter, Index 1 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCACCAAATCGCGTATG2373010.7185138592049413TruSeq Adapter, Index 1 (97% over 38bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2032740.6154849166924086No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCACCAAATCTCGTATT1985430.601160117943568TruSeq Adapter, Index 1 (97% over 38bp)
CTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGCGTCCTCCCTCGGGAGGG1265920.38330266819133474No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCACCAAATCTCGGTTG1263120.38245486780036553TruSeq Adapter, Index 1 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCACCAAATCGCGTTTG1043830.31605695789478083TruSeq Adapter, Index 1 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCACCAAATCGCGGATG737230.22322281508365274TruSeq Adapter, Index 1 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCACCAAATCTCGTTTT654190.19807947777433746TruSeq Adapter, Index 1 (97% over 38bp)
CCCCTCCCGGCGCCCACCCCCGCGGGGAATCCCCCGCGAGGGGGGTCTCCC595290.18024539097859238No Hit
CCCCCCTCCCGGCGCCCACCCCCGCGGGGAATCCCCCGCGAGGGGGGTCTC573670.17369916081689443No Hit
CCCCGACCCGCGCGCCCTCCCGAGGGAGGACGCGGGGCCGGGGGGCGGAGA553280.16752535725551512No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT485760.14708125639900055No Hit
CCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGCGTCCTCCCTCGGGAGG457630.13856389032829922No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCACCAAATCGCGGTTG395940.11988503100012408TruSeq Adapter, Index 1 (97% over 38bp)
CTCCCCCCTCCCGGCGCCCACCCCCGCGGGGAATCCCCCGCGAGGGGGGTC330640.10011311473930654No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)