FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005204301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005204301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33026642
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCGACCCGCGCGCCCTCCCGAGGGAGGACGCGGGGCCGGGGGGCGGAGA1383810.41899809250967746No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTTTTTTTTTTTT881630.26694509238935044No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA635350.19237499228653038No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT611960.1852928311633983No Hit
CTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGCGTCCTCCCTCGGGAGGG531920.16105785141583573No Hit
CGTCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCC499600.15127181261721975No Hit
CGCCCCCGCGGGGGAGACCCCCCTCGCGGGGGATTCCCCGCGGGGGTGGGC407970.12352754482275248No Hit
CCCGACCCGCGCGCCCTCCCGAGGGAGGACGCGGGGCCGGGGGGCGGAGAC389880.11805014872538358No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG358990.10869709369786973No Hit
GTCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCC335700.10164521116012945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGAC254350.025.7614691
GGGGTTT2689350.023.62027428
GGGTTTT2508100.023.43265229
GGGGGTT2900200.022.60122727
GCGGAGA295050.021.61167545
CCGACCC306800.021.2813223
CGACCCG313800.020.7277284
ACCCGCG319700.020.295946
GACCCGC321500.020.2732835
TCCGCCG38400.019.9283772
CCCGACC335150.019.535692
ACGCGGG328750.019.45677830
GACGCGG329700.019.4075429
GCGCCCT344000.018.83034112
CCGAGGG341050.018.62961420
CCCGCGC351550.018.604357
CGAGGGA340900.018.55201321
CCCGAGG343500.018.45744119
CGCCCTC354300.018.32101813
GGGCGGA369300.018.05855843
Produced by FastQC (version 0.11.5)