FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005204389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005204389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40573075
Sequences flagged as poor quality0
Sequence length35-51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC1598520.3939854201339189No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT894870.22055759885096213No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTC518440.1277793216314021No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCG246400.031.419063
CCGGACA261600.029.66160412
CACCCGG268750.029.0296444
CCCGGAC268250.028.97601511
GCCCGGA270450.028.7894210
CGGACAC270200.028.717113
ACCCGGC275500.028.3859635
GGACACG276250.028.09513514
GACACGG276850.028.00028415
ACACGGA281050.027.62777516
CGGACAG282500.027.32543619
ACGGACA283750.027.30808318
CACGGAC286750.027.05446617
CTCACCC306750.025.5674862
CCTCACC336750.023.8488921
CTTTCTC512750.023.5865645
GATTGAC330700.023.52273826
CGGCCCG347950.022.5047058
GGACAGG351600.022.18181220
GGATTGA353900.022.0371125
Produced by FastQC (version 0.11.5)