FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005204391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005204391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31049596
Sequences flagged as poor quality0
Sequence length35-51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG755270.2432463211437598No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT618240.1991137018336728No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGG606010.19517484221050735No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC578580.1863405887793194No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTC482530.1554062088279667No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT434650.13998571833269585No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGC431100.13884238622621692No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTT427910.1378149976572964No Hit
CCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTC385600.12418841134036011No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG344230.11086456648260415No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTT342790.11040079233237043No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCG342430.11028484879481201No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT341180.10988226706717859No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCG97700.028.7524783
CGGTGGC251050.028.5761281
CCGGACA99600.028.32439612
CGGACAC101900.027.6190413
ACGGACA106100.026.7926318
CGGACAG108150.026.22171219
CACCCGG109550.025.9061374
ACACGGA109500.025.84102416
CCCGGAC109900.025.77113311
GACACGG109600.025.75742515
GGACACG109550.025.68937714
GCCCGGA110600.025.54709410
ACCCGGC111000.025.5099895
CACGGAC111550.025.4847317
GGCGATC32550.024.0758252
GGTGGCG298000.023.929312
GATTGAC133950.021.1193126
TCTAAGT226100.020.42179345
TGGCGCG351750.020.2055024
GCCAGTA149650.019.9609244
Produced by FastQC (version 0.11.5)