FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005204434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005204434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29290793
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATAGAGTATCTCGTATG534215118.23832833750865TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATAGAGTATCGCGTATG7202252.4588784605456055TruSeq Adapter, Index 7 (97% over 37bp)
CCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGC6902692.356607415852483No Hit
CCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCA6509632.222415077666214No Hit
CTCCCATCCAAGTACTAACCAGGCCCGACCCTGCTTAGCTTCCGAGATCAG5772911.970895769192729No Hit
GTTAAGACTCAGAGTTGTTCCTCTCCACGGAAATCTTTAGTAAAAGGCGAA5392371.8409778116966653No Hit
ATTGGGTTAAGACTCAGAGTTGTTCCTCTCCACGGAAATCTTTAGTAAAAG4002791.3665693516730666No Hit
CCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCA3735131.275189101230547No Hit
ACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTC3298611.1261593361436135No Hit
GTGCAGTAGTGAGAAGGGGGGAAAGAGTAGAACAAGGAGTTCGATCTGTAA3215011.0976179443144471No Hit
CTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGG3210901.0962147730175826No Hit
GTCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAAT2870690.9800656472496323No Hit
GTCAAGTGCAGTAGTGAGAAGGGGGGAAAGAGTAGAACAAGGAGTTCGATC2355380.8041366445763349No Hit
CCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCG1983500.6771752475257328No Hit
GGTTAAGACTCAGAGTTGTTCCTCTCCACGGAAATCTTTAGTAAAAGGCGA1904080.6500609252880248No Hit
GCCTACAGCACCCGGTATTCCCAGGCGGTCTCCCATCCAAGTACTAACCAG1867670.6376303980571643No Hit
GGCTAGTCAAGTGAAGCAGTGGGAGTGGAGAAGGAACAAAGAAATCTGTAA1812960.6189521738110675No Hit
CTCAAAAAATTGGGTTAAGACTCAGAGTTGTTCCTCTCCACGGAAATCTTT1585920.5414397623171213No Hit
CTCCTATTCCATCTCCCTGCTCCAAAAATCCATTTAATATATTGTCCTCGG1527940.5216451463092856No Hit
CCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGTGCAATGGATA1516520.5177463102484116No Hit
GGCCTCAAAAAATTGGGTTAAGACTCAGAGTTGTTCCTCTCCACGGAAATC1508610.5150458029593121No Hit
GCAGTAGTGAGAAGGGGGGAAAGAGTAGAACAAGGAGTTCGATCTGTAACT1442770.4925677498728014No Hit
CATCCAAGTACTAACCAGGCCCGACCCTGCTTAGCTTCCGAGATCAGACGA1407170.4804137600508119No Hit
GGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCCTGCTCC1258540.42967085254400594No Hit
TGGGTTAAGACTCAGAGTTGTTCCTCTCCACGGAAATCTTTAGTAAAAGGC1204390.4111838146546596No Hit
CACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAG1164770.397657379914569No Hit
CCCGGTATTCCCAGGCGGTCTCCCATCCAAGTACTAACCAGGCCCGACCCT915690.3126204196656608No Hit
AGCCAGTCAAATTTAGCAGTGGGGGGTTGTATACCAACTTTAGTGACACTA901760.3078646590414947No Hit
CTAGTCAAGTGCAGTAGTGAGAAGGGGGGAAAGAGTAGAACAAGGAGTTCG896320.306007420147348No Hit
GTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCCTGCTCCA881650.30099902040890464No Hit
GCCAGTCAAATTTAGCAGTGGGGGGTTGTATACCAACTTTAGTGACACTAA865910.2956253181673846No Hit
CCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGTGCAATGGATAA856270.2923341815976099No Hit
ACCAGGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCCTG853840.29150456937099656No Hit
CTAGTCAAGTGAAGCAGTGGGAGTGGAGAAGGAACAAAGAAATCTGTAACT831010.28371031129133306No Hit
GTCAAATTTAGCAGTGGGGGGTTGTATACCAACTTTAGTGACACTAATGTT789870.26966494215434866No Hit
CTCAAAAAATTAGCTTAAGACTCAGAGTTGTTCCTCTCCACGGAAATCTTT769580.2627378507642316No Hit
CCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAG758150.25883560066127265No Hit
ATCCAAGTACTAACCAGGCCCGACCCTGCTTAGCTTCCGAGATCAGACGAG752400.25687252646249625No Hit
GCCTCAAAAAATTGGGTTAAGACTCAGAGTTGTTCCTCTCCACGGAAATCT745870.25464315698110324No Hit
GTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGTG728060.2485627480280237No Hit
GGGGGAAAGAGTAGAACAAGGAGTTCGATCTGTAACTGACTGTGAACAATC695490.2374432129577373No Hit
CCGGTATTCCCAGGCGGTCTCCCATCCAAGTACTAACCAGGCCCGACCCTG690140.23561670044235403No Hit
CAGGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCCTGCT688380.23501582903542423No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT645470.2203661744494251No Hit
CCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATC627010.2140638527608317No Hit
CCCAGGCGGTCTCCCATCCAAGTACTAACCAGGCCCGACCCTGCTTAGCTT626790.21398874383496547No Hit
CAAAAAATTGGGTTAAGACTCAGAGTTGTTCCTCTCCACGGAAATCTTTAG618290.21108680806286123No Hit
GGCCTCAAAAAATTAGCTTAAGACTCAGAGTTGTTCCTCTCCACGGAAATC614710.20986458099649263No Hit
GTCAAGTGAAGCAGTGGGAGTGGAGAAGGAACAAAGAAATCTGTAACTGGT608590.2077751872405776No Hit
CCCATCCAAGTACTAACCAGGCCCGACCCTGCTTAGCTTCCGAGATCAGAC598500.20433041877698566No Hit
CACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGG589530.20126802302689448No Hit
AGTCAAATTTAGCAGTGGGGGGTTGTATACCAACTTTAGTGACACTAATGT515430.1759699711783153No Hit
CAAGTGCAGTAGTGAGAAGGGGGGAAAGAGTAGAACAAGGAGTTCGATCTG512730.17504817981541163No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG510430.17426295013590107No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC496970.1696676494897219No Hit
ATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCG483520.16507576288562759No Hit
GGGTTAAGACTCAGAGTTGTTCCTCTCCACGGAAATCTTTAGTAAAAGGCG452550.15450247454891372No Hit
GTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCTATTTCCA451600.154178140550855No Hit
GCCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTC446050.15228334719377518No Hit
GACTAGTCAAGTGCAGTAGTGAGAAGGGGGGAAAGAGTAGAACAAGGAGTT444520.15176099875479643No Hit
AGTCAAGTGAAGCAGTGGGAGTGGAGAAGGAACAAAGAAATCTGTAACTGG437090.14922436548576887No Hit
GGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCTATTTCC436730.14910145997071503No Hit
GCTCCTATTCCATCTCCCTGCTCCAAAAATCCATTTAATATATTGTCCTCG433980.14816259839738719No Hit
CCTGGAGGTACTGCAATACCAGGTCGATGCGTGGAGTGGACGGAGCAAGCT432760.14774608526303812No Hit
CTACAGCACCCGGTATTCCCAGGCGGTCTCCCATCCAAGTACTAACCAGGC430150.14685502027889788No Hit
GTCAAAAATTGCCAATGCCGACTATATTTCAAGTCGTCATGGCGGGGTATT429230.14654092840709365No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTC424270.14484756353301873No Hit
CCAGGCGGTCTCCCATCCAAGTACTAACCAGGCCCGACCCTGCTTAGCTTC420020.1433965956469666No Hit
CTCCCGTCCAAGTACTAACCAGGCCCGACCCTGCTTAGCTTCCGAGATCAG414520.14151887250031095No Hit
TGCAGTAGTGAGAAGGGGGGAAAGAGTAGAACAAGGAGTTCGATCTGTAAC401980.137237663725936No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGAT398150.13593008560744668No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA378630.12926587545786145No Hit
ATTCCATCTCCCTGCTCCAAAAATCCATTTAATATATTGTCCTCGGATAGA377820.12898933804899032No Hit
CAGTCAAATTTAGCAGTGGGGGGTTGTATACCAACTTTAGTGACACTAATG377660.1289347133756331No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC371830.12694432684017806No Hit
CAGGCGGTCTCCCATCCAAGTACTAACCAGGCCCGACCCTGCTTAGCTTCC359370.12269043040248177No Hit
GTTTCAGACTCACACTCGTTCCTCTCCACGGAAATCTTTAGTAAAAGGCGA355260.12128725910561726No Hit
CTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAA345500.11795515403082463No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA342430.11690704311078227No Hit
GCCTCAAAAAATTAGCTTAAGACTCAGAGTTGTTCCTCTCCACGGAAATCT338350.11551411394017225No Hit
CCAAGTACTAACCAGGCCCGACCCTGCTTAGCTTCCGAGATCAGACGAGAT337860.11534682587801565No Hit
GTGAGAAGGGGGGAAAGAGTAGAACAAGGAGTTCGATCTGTAACTGACTGT331800.11327791637460959No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT329370.11244830414799625No Hit
TTGGGTTAAGACTCAGAGTTGTTCCTCTCCACGGAAATCTTTAGTAAAAGG328210.11205227526615616No Hit
CCTCAAAAAATTGGGTTAAGACTCAGAGTTGTTCCTCTCCACGGAAATCTT326670.11152651278509257No Hit
CTCCTATTTCCAAAAATCCATTTAATATATTGTCCTCGGATAGAGGACGTA323920.11058765121176473No Hit
GCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGA322450.11008578702529494No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG311770.10643959007869812No Hit
CCCCGCTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGC301200.10283094759503439No Hit
GGCCTCAAAAAATTAGTTTAAGACTCAGAGTTGTTCCTCTCCACGGAAATC299810.10235639574524323No Hit
GCTAGTCAAGTGAAGCAGTGGGAGTGGAGAAGGAACAAAGAAATCTGTAAC299400.10221642001976526No Hit
TCAAAAAATTGGGTTAAGACTCAGAGTTGTTCCTCTCCACGGAAATCTTTA298420.10188184389545206No Hit
TAAGACTCAGAGTTGTTCCTCTCCACGGAAATCTTTAGTAAAAGGCGAAAG295960.10104198954258424No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)