FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005204435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005204435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29290793
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCAGATCGCATAAATCTTTCGCCTTTTACTAAAGATTTCCGTGGAGAGG14682175.012554627660644No Hit
CCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCC6299342.1506211866643556No Hit
GGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCAT4980831.7004763237376332No Hit
CACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGG3301761.1272347594003345No Hit
GGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCA3009331.0273979267136948No Hit
CCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCG2797000.9549075711265311No Hit
ACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTC2722360.9294251610053712No Hit
GGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATT2436650.8318825645997362No Hit
GTAGTGAGTTATCTCAATTGATTGTTCACAGTCAGTTACAGATCGAACTCC2289490.7816415212794No Hit
AGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCC2260590.7717749396542456No Hit
AAGGTAGTGAGTTATCTCAATTGATTGTTCACAGTCAGTTACAGATCGAAC2044860.6981238097582404No Hit
ATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCGG2038790.6960514862127496No Hit
CGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCGGATGTGCT1981070.6763456352991195No Hit
ACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTC1920980.655830656411385No Hit
CCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGC1907010.6510612396188796No Hit
GTGAGTTATCTCAATTGATTGTTCACAGTCAGTTACAGATCGAACTCCTTG1892800.6462098858163383No Hit
TTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCGTGGAGAGGA1839200.6279106202416576No Hit
ATCTGTTCTTATCAGTTTAATATCTGATACGTCCTCTATCCGAGGACAATA1788560.610621911124086No Hit
GGCTTATCCATTGCACTCCGGATGTGCTGACCCCTGCGATTTCCCCAAATG1634860.558148084280272No Hit
CATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCG1620840.5533615972773424No Hit
TGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCGGA1604750.5478684035628534No Hit
CCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCC1576300.5381554538315163No Hit
TTCAGATCGCATAAATCTTTCGCCTTTTACTAAAGATTTCCGTGGAGAGGA1540390.5258956287048971No Hit
GTGCAGTGGTGTTTACAACTAATTGATCACAACCAGTTACAGATTTCTTTG1367650.4669214657315696No Hit
CGAAGGTAGTGAGTTATCTCAATTGATTGTTCACAGTCAGTTACAGATCGA1272440.4344163710419175No Hit
CTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCGTGGAGAGG1208720.4126620948773903No Hit
GATCAAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATACGTCCTCTA1049100.3581671551193578No Hit
AGTGCAGTGGTGTTTACAACTAATTGATCACAACCAGTTACAGATTTCTTT846000.288827960376491No Hit
ATCAAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATACGTCCTCTAT838440.28624694456036065No Hit
GATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCA826900.2823071399944686No Hit
GTTTAATATCTGATACGTCCTCTATCCGAGGACAATATATTAAATGGATTT817790.2791969476551898No Hit
ATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCAC797470.27225961413881833No Hit
CTGTTCTTATCAGTTTAATATCTGATACGTCCTCTATCCGAGGACAATATA757460.25860003175741947No Hit
CTCTTCAGATCGCATAAATCTTTCGCCTTTTACTAAAGATTTCCGTGGAGA753440.25722758683931846No Hit
AGGTAGTGAGTTATCTCAATTGATTGTTCACAGTCAGTTACAGATCGAACT727490.24836814762918846No Hit
GGTAGTGAGTTATCTCAATTGATTGTTCACAGTCAGTTACAGATCGAACTC724320.24728589628829784No Hit
CCCAGGGCGAGGCTTATCCATTGCACTCCGGATGTGCTGACCCCTGCGATT705550.24087773929507475No Hit
AGTGAGTTATCTCAATTGATTGTTCACAGTCAGTTACAGATCGAACTCCTT698990.23863812768742726No Hit
CAGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATC671580.22928023833291233No Hit
GCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTAGTA669810.22867595288389767No Hit
CTAAGATCAAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATACGTCC663700.22658997317006746No Hit
CATAAATCTTTCGCCTTTTACTAAAGATTTCCGTGGAGAGGAACAACTCTG637310.21758031610820505No Hit
CAAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATACGTCCTCTATCC626820.21399898596121997No Hit
GTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTAGTACTTGGATGGG625290.2134766375222412No Hit
GTTATCTCAATTGATTGTTCACAGTCAGTTACAGATCGAACTCCTTGTTCT611530.20877891561351716No Hit
CTACAATATAGCTGCTAAGTGCTGTGTTGTCGTTCCCCCTGCTTAAAATAA600890.2051463748352597No Hit
GAAGGTAGTGAGTTATCTCAATTGATTGTTCACAGTCAGTTACAGATCGAA598940.20448063662871813No Hit
GTTCTTATCAGTTTAATATCTGATACGTCCTCTATCCGAGGACAATATATT581970.19868700721076415No Hit
CTTCAAACCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCGTGGAGAGG565780.1931596730754268No Hit
ATGGTAGTGGGTTATCAGAACTTATTAACATTAGTGTCACTAAAGTTGGTA565580.19309139223373023No Hit
TGGTAGTGGGTTATCAGAACTTATTAACATTAGTGTCACTAAAGTTGGTAT561440.19167797881061124No Hit
CGCATAAATCTTTCGCCTTTTACTAAAGATTTCCGTGGAGAGGAACAACTC557450.19031577601876468No Hit
TACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACT529190.18066769308703934No Hit
CACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCGGATGTG523290.1786534082569905No Hit
CTTATCCATTGCACTCCGGATGTGCTGACCCCTGCGATTTCCCCAAATGTG521700.17811057556550278No Hit
TACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGT519960.17751653224274264No Hit
GGTGTTTACAACTAATTGATCACAACCAGTTACAGATTTCTTTGTTCCTTC514080.17550907549686345No Hit
CGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTAGTACTT490330.16740072554539578No Hit
GAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTG478770.16345409289533402No Hit
CTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTAGTACTTGGAT463730.15831937359975198No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA445040.1519385289432075No Hit
GCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCA443310.15134789966253218No Hit
AAATCTTTCGCCTTTTACTAAAGATTTCCGTGGAGAGGAACAACTCTGAGT440080.15024516406913258No Hit
ATAAATCTTTCGCCTTTTACTAAAGATTTCCGTGGAGAGGAACAACTCTGA434310.14827526178618652No Hit
CCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAG431630.1473602985074525No Hit
GGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCGGATGTGCTGACC429020.14646923352331226No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA423870.1447110018496256No Hit
TAGTGAGTTATCTCAATTGATTGTTCACAGTCAGTTACAGATCGAACTCCT418810.14298349655470236No Hit
ACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGG410800.14024884884475472No Hit
GATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCGGAT410570.14017032587680367No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTGTTTTTTTTTTTT400750.13681773654950208No Hit
CTGATACGTCCTCTATCCGAGGACAATATATTAAATGGATTTTTGGAGCAG393140.1342196505229476No Hit
CAGAACTTATTAACATTAGTGTCACTAAAGTTGGTATACAACCCCCCACTG376310.12847381769418126No Hit
GTGGGTTATCAGAACTTATTAACATTAGTGTCACTAAAGTTGGTATACAAC373990.12768175993050104No Hit
ATCTGATACGTCCTCTATCCGAGGACAATATATTAAATGGATTTTTGGAGC359840.1228508903804687No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTTTTTTTTTTTT352650.12039619412147701No Hit
CTGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTAGTACTTGGATGGGAG347220.11854236926941512No Hit
ATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCGGATG341100.1164529755135001No Hit
CGATGGTAGTGGGTTATCAGAACTTATTAACATTAGTGTCACTAAAGTTGG330460.11282043473524257No Hit
GTGGTGTTTACAACTAATTGATCACAACCAGTTACAGATTTCTTTGTTCCT329720.11256779562096525No Hit
AAGATCAAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATACGTCCTC322600.11013699765656737No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGC313030.1068697593813865No Hit
GGGTTATCAGAACTTATTAACATTAGTGTCACTAAAGTTGGTATACAACCC310990.10617329479608148No Hit
GTGTAGTATCTGTTCTTATCAGTTTAATATCTGATACGTCCTCTATCCGAG310130.10587968717678624No Hit
CAGATCGCATAAATCTTTCGCCTTTTACTAAAGATTTCCGTGGAGAGGAAC295640.10093274019586974No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACCG650.045.0621871
CTTCAGA1823250.041.2881621
AGATCGC1883450.036.8707245
GATCGCA1906700.036.4329266
TCGCATA1919400.036.1877338
ATCGCAT1920250.036.16537
GCCATAC58550.035.4802171
CGCATAA1978850.035.125219
TCGTGTA35500.034.9845115
CGGTGGC129650.034.895831
GCATAAA1995400.034.83951610
TTCAGAT2178150.034.5041162
TCAGATC2186100.034.362823
AACCGTA87100.034.1999326
CCGTATA87700.033.9921268
CATAAAT2066000.033.6881711
CAAACCG89700.033.6601074
ACCGTAT88450.033.6525047
TACGCGT551.12990165E-732.72533835
CGTAATT9500.032.255041
Produced by FastQC (version 0.11.5)