FastQCFastQC Report
Wed 8 May 2024
EGAF00005204438

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005204438
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27043024
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAATATATCTCGTAT831336030.74123663093299TruSeq Adapter, Index 19 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAATATATCGCGTAT12237934.5253555963268015TruSeq Adapter, Index 19 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAATATATCTCGTA6921402.5594031200061056TruSeq Adapter, Index 19 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAATATATCGCGTA2352010.8697289178902479TruSeq Adapter, Index 19 (97% over 38bp)
CCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTC1056210.39056652835866285No Hit
CCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCG1006380.3721403346016333No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT999360.369544471062112No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT862160.31881049989084065No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT861740.3186551918158265No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA825470.30524323019496635No Hit
CCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGC787730.2912876903115569No Hit
GTTAAGACTCAGAGTTGTTCCTCTCCACGGAAATCTTTAGTAAAAGGCGA784550.29011178631502155No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC766700.28351119312692247No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT738010.2729021724789358No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG677960.2506968155632299No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA630700.23322095931283424No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT596110.22043022999203046No Hit
GTGCAGTAGTGAGAAGGGGGGAAAGAGTAGAACAAGGAGTTCGATCTGTA555730.2054984679228181No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA555240.20531727516863496No Hit
GTCAAATTTAGCAGTGGGGGGTTGTATACCAACTTTAGTGACACTAATGT524970.19412400033369048No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG514170.1901303641190423No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC512200.189401895291No Hit
GTCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAA485740.17961748656511195No Hit
CTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGG464090.17161172507926628No Hit
GTCAAGTGCAGTAGTGAGAAGGGGGGAAAGAGTAGAACAAGGAGTTCGAT444160.16424198713871643No Hit
ACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGT442570.1636540351404488No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG440200.1627776538600121No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT433140.16016699907525134No Hit
GCCAGTCAAATTTAGCAGTGGGGGGTTGTATACCAACTTTAGTGACACTA432480.15992294352880063No Hit
ATTGGGTTAAGACTCAGAGTTGTTCCTCTCCACGGAAATCTTTAGTAAAA388350.14360450221839097No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG385560.14257281286294018No Hit
AGCCAGTCAAATTTAGCAGTGGGGGGTTGTATACCAACTTTAGTGACACT381040.1409014021508837No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA313970.11610018169565652No Hit
GGCTAGTCAAGTGAAGCAGTGGGAGTGGAGAAGGAACAAAGAAATCTGTA313480.1159189889414734No Hit
CTCAAAAAATTAGCTTAAGACTCAGAGTTGTTCCTCTCCACGGAAATCTT295300.10919636798014896No Hit
GCAGTAGTGAGAAGGGGGGAAAGAGTAGAACAAGGAGTTCGATCTGTAAC295230.10917048330097996No Hit
AGTCAAATTTAGCAGTGGGGGGTTGTATACCAACTTTAGTGACACTAATG287480.1063046795358389No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC270700.10009975215789478No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)