FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005204438

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005204438
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27043024
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAATATATCTCGTATG831275730.739006850713146TruSeq Adapter, Index 19 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAATATATCGCGTATG12236794.524934045837478TruSeq Adapter, Index 19 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAATATATCTCGTAT6920412.55903703668643TruSeq Adapter, Index 19 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAATATATCGCGTAT2351520.8695477251360646TruSeq Adapter, Index 19 (97% over 38bp)
CCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCA1055810.3904186159062685No Hit
CCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGC1006290.3721070542998446No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT998680.3692930198930416No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTG861930.31872545023071386No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG857690.3171575782353334No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGAT825240.30515818053483956No Hit
GTTAAGACTCAGAGTTGTTCCTCTCCACGGAAATCTTTAGTAAAAGGCGAA784170.2899712694852469No Hit
CCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCA782730.28943878465662715No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA766450.283418747844176No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTC737960.2728836834223865No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC677910.2506783265066806No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC630640.23319877244497508No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC596050.2204080431241713No Hit
GTGCAGTAGTGAGAAGGGGGGAAAGAGTAGAACAAGGAGTTCGATCTGTAA555620.20545779199840963No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG555160.2052876926781561No Hit
GTCAAATTTAGCAGTGGGGGGTTGTATACCAACTTTAGTGACACTAATGTT524600.19398718131522572No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA514000.1900675013267747No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCT511680.1892096091028873No Hit
GTCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAAT485330.1794658763014077No Hit
CTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGG464020.17158584040009728No Hit
GTCAAGTGCAGTAGTGAGAAGGGGGGAAAGAGTAGAACAAGGAGTTCGATC444100.16421980027085728No Hit
ACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTC442520.1636355460838995No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT439900.16266671952071632No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC433000.1601152297169133No Hit
GCCAGTCAAATTTAGCAGTGGGGGGTTGTATACCAACTTTAGTGACACTAA432070.15977133326509638No Hit
ATTGGGTTAAGACTCAGAGTTGTTCCTCTCCACGGAAATCTTTAGTAAAAG388350.14360450221839097No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGC385550.1425691150516303No Hit
AGCCAGTCAAATTTAGCAGTGGGGGGTTGTATACCAACTTTAGTGACACTA380960.14087181966040482No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCAC313920.11608169263910723No Hit
GGCTAGTCAAGTGAAGCAGTGGGAGTGGAGAAGGAACAAAGAAATCTGTAA313370.11587831301706494No Hit
CTCAAAAAATTAGCTTAAGACTCAGAGTTGTTCCTCTCCACGGAAATCTTT295180.10915199424443066No Hit
GCAGTAGTGAGAAGGGGGGAAAGAGTAGAACAAGGAGTTCGATCTGTAACT295120.10912980737657149No Hit
AGTCAAATTTAGCAGTGGGGGGTTGTATACCAACTTTAGTGACACTAATGT287180.1061937451965431No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCC270700.10009975215789478No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTCG207.0374284E-444.99828718
TCGTATG8580300.044.5676245
GCGTATG1306100.043.59604645
AGATCGG1044800.041.823321
TATCTCG9329400.040.9705741
TCTCGTA9332050.040.95034843
ATCTCGT9336150.040.94417642
TATATCT9334950.040.93456639
ATATCTC9351450.040.8607340
ACACGTC10877100.040.77325413
GCACACG10899150.040.76693711
CACACGT10888450.040.76587712
CTCGTAT9384450.040.74752844
CACGTCT10877750.040.7292414
ATCGGAA10932700.040.7196242
GATCGGA10937750.040.7162631
AGAGCAC10934900.040.7058758
ACGTCTG10875750.040.7009415
TCGGAAG10952400.040.6988643
AGCACAC10925000.040.6976610
Produced by FastQC (version 0.11.5)