FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005204439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005204439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27043024
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTTTTTTTTTTTT13199344.880866873467997No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGAATGAGAGTGTAGATCT7271652.6889189611339326Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGTTTTTTTTTTTTTTTTTT4238961.567487423004173No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTTTTTTTTTTTTT3034061.1219381382792102No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGTTTTTTTTTTTTTTTTTTTT2006760.742061982417351No Hit
CTTCAGATCGCATAAATCTTTCGCCTTTTACTAAAGATTTCCGTGGAGAGG1887930.6981208906222913No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTTTTTTTTTTT1780400.6583583256073728No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTGTTTTTTTTTTTTTTTTT1232270.45567019428004796No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTTTTTTTTTTTTTT1224020.4526194999494139No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGAATGAGAGTGTAGTTCT1221090.45153604123562513Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTTTGTTTTTTTT1082670.40035093708455094No Hit
CCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCC1025310.3791402914111972No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTTTTGTTTTTTT876600.3241501394222776No Hit
GGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCAT846240.3129235842855444No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA753320.27856352159433057No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTGTTTTTTTTTTTTTTTTTT700130.25889486323718824No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGTTTTTTTTTGTTTTTTTT683820.25286373299080755No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA646880.23920401801218683No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT569690.21066061251138185No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGTTTTTTTTTTGTTTTTTT569260.2105016066250579No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGC567510.2098544896458325No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGTGTTTTTTTTTTTTTTTTTT554500.2050436371317054No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGTTTTTTTTTTTTTTTTTTTT545760.20181175004688823No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGTTTTTTTTTTTTTTTTTTTT531280.1964573192702118No Hit
TTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCGTGGAGAGGA524980.19412769814500036No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTTTTTTTTTT496120.18345581470474603No Hit
GGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCA486830.18002054799788664No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGTTTTTTTTTTTTTTTTT485010.1793475463394922No Hit
GTAGTGAGTTATCTCAATTGATTGTTCACAGTCAGTTACAGATCGAACTCC433470.1602890268484767No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGTTTTTTTTTTTTTTTTTG414800.15338521313296916No Hit
AGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCC396680.14668477903950386No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTC384670.14224370765636268No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGTTTTTTTTTTTTTTTTTTT382420.14141170011164433No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT382210.14133404607413727No Hit
GTGAGTTATCTCAATTGATTGTTCACAGTCAGTTACAGATCGAACTCCTTG380870.14083853935861612No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTTTTTTTTTTTG377230.13949253604182726No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGTTTTTTTTTTTTTTTTT376340.1391634308352498No Hit
CCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTC372950.13790987280120745No Hit
GGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATT371960.13754378948153137No Hit
TGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCGGA360230.13320625681506626No Hit
ACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTC352380.1303034749368266No Hit
CACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAG349230.12913866437422086No Hit
AAGGTAGTGAGTTATCTCAATTGATTGTTCACAGTCAGTTACAGATCGAAC342810.12676466951329113No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATC339910.1256923042334319No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTTGTTTTTTTTT336220.12432781186009374No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGTTTTTTTTTTTTTTTTTTT326240.12063739617285404No Hit
CGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCGGATGTGCT315720.11674729867488193No Hit
CAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGT310860.11495016237829023No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTGTTTTTTTTTTTT307920.11386300585319158No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGTTTTTTTTTTTTTTTTTTTT305110.11282392087512107No Hit
ATCTGTTCTTATCAGTTTAATATCTGATACGTCCTCTATCCGAGGACAATA299170.11062742095706457No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGTTTTTGTTTTTTTTTTTT296290.10956245129982506No Hit
CATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCG285040.10540241357623319No Hit
ATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCGG277570.10264014852776819No Hit
GATCAAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATACGTCCTCTA274570.10153080513481036No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)