FastQCFastQC Report
Wed 11 May 2022
EGAF00005204469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005204469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67299785
Sequences flagged as poor quality0
Sequence length35-51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGT6286500.9341040242550552No Hit
CGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAA3923090.5829275680449797No Hit
CTCGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAA3660600.5439244716755038No Hit
CTGACTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCAT2927290.43496275656749867No Hit
CCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTA2718640.40395968575531116No Hit
ACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGA2650700.39386455692243294No Hit
CTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTC2566730.38138754826631316No Hit
CGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTT2409220.357983313022471No Hit
CTCAGACTTTATTCAAAGACCAGGAAGGGCCGGTGCAAGGAGGGGAGGAGG2314420.3438970867440364No Hit
CCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGA2295800.3411303617091793No Hit
CCTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCT2284660.33947508153257844No Hit
CTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGTGCGTCGGG2241040.332993634377881No Hit
CTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGG2145060.31873207321538993No Hit
CTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAAC2135530.31731602114330676No Hit
CTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCGTGAGG1870790.27797859978304534No Hit
CGAAACCCCGACCCAGAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTT1813880.2695224063494408No Hit
CTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCCGCCAAGCC1793340.26647039065577993No Hit
CACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCA1773930.2635862804019359No Hit
CAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATGGGAATAACGC1753060.2604852303762932No Hit
CTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGACTTCC1752190.26035595804652273No Hit
CTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGGG1576780.23429198176487487No Hit
CTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCT1572170.2336069870059763No Hit
CAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTA1556420.23126671207047692No Hit
CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGC1489470.22131868623354442No Hit
CAAAAGGTCAGAAGGATCGTGAGGCCCCGCTTTCACGGTCTGTATTCGTA1448840.2152815198443799No Hit
CATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCAC1424940.21173024549781252No Hit
CCCCGACCCAGAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTTCCCC1422840.2114182088397459No Hit
CTCAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGG1421550.21122652917836215No Hit
CCGAGGCCAACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGG1416520.21047912708785027No Hit
GTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGC1404940.20875846780194024No Hit
CTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCATTGG1385480.20586692810385648No Hit
CGCAGGGCCGGCGGACCCCGCCCCGGGCCCCTCGCGGGGACACCGGGGGGG1379980.20504968923749164No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCA1351830.20086691213055136No Hit
CTCCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGC1332100.19793525343357338No Hit
CTTGTGTCGAGGGCTGACTTTCAATAGATCGCAGCGAGGGAGCTGCTCTG1261550.1874523076113839No Hit
GTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCAC1254760.18644338908363525No Hit
CTTGGAGACCTGCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACC1252120.1860511144277801No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGA1251220.18591738443146588No Hit
CTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCATCC1225240.18205704520452776No Hit
CTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGCCGAG1213030.18024277492119775No Hit
CGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCC1196350.17776431232284026No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA1179490.17525910372521994No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA1162780.17277618346031862No Hit
CTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTA1112920.165367541664509No Hit
CAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAAC1096060.16286233306688871No Hit
CTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCACGGGAGGTTT1074960.15972710759774345No Hit
CCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAA1074280.1596260671560838No Hit
CGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCTT1049920.15600644192251134No Hit
CTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTT1029420.15296036978424227No Hit
CAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCATGGC1021380.1517657151505016No Hit
GTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCGACCC1004960.14932588566219046No Hit
GGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTAT997200.14817283591619201No Hit
GTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCATGGCAACAA993090.14756213559969025No Hit
ACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCCGCCAAGC988410.14686673961885616No Hit
CCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCG982370.1459692627547027No Hit
CGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCTATATCAACCAACAC976940.14516242511027339No Hit
CTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTT965200.14341799160279636No Hit
CACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCATG948150.14088455111706524No Hit
CTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCAT934260.13882065150728193No Hit
CTGTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTC932650.13858142340276422No Hit
CAAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGCAGCGAGGGAG920040.13670771756551675No Hit
GTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATT916250.13614456569214894No Hit
CCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACTAGAGTC903300.13422033963407165No Hit
GTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGATGACGAGG902520.13410444030393262No Hit
TTTCAATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTACGAAACCCCGA899790.13369879264844606No Hit
CAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCCAGTTATCC886990.1317968549230878No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA884440.13141795326686406No Hit
CGTGAGGCCCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGATCA883380.13126044904898285No Hit
CTCTCGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGT880600.13084737194925658No Hit
CCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATT879440.130675008842896No Hit
CTTATCCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGT868360.12902864399938277No Hit
GGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGT861170.12796028991771666No Hit
CGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCC852840.12672254450738588No Hit
CTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGG852300.12664230650959732No Hit
CGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCA850630.12639416307199197No Hit
CCAAAAGGTCAGAAGGATCGTGAGGCCCCGCTTTCACGGTCTGTATTCGT848650.12609995708010063No Hit
GTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGC842450.12517870599438022No Hit
ACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCATGG841890.1250954962188958No Hit
CGAGGGCTGACTTTCAATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTA836640.12431540457372933No Hit
CTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCG833770.12388895447437165No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCG825070.12259623117666721No Hit
CGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCG817690.12149964520689033No Hit
CTCCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCGCCCATCTC812940.12079384800412066No Hit
GCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAA809950.12034956723858777No Hit
CTCGTTCATCCATTCATGCGCGTCACTAATTAGATGACGAGGCATTTGG809290.12025149857462399No Hit
CCTCGCGGCGCCCATCTCCGCCACTCCGGATTCGGGGATCTGAACCCGA803090.11933024748890357No Hit
CTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCA796640.11837184918198475No Hit
CTCGCGGCGCCCATCTCCGCCACTCCGGATTCGGGGATCTGAACCCGACT793410.11789190708410138No Hit
CGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAAT789860.11736441654308406No Hit
CCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCGCCCATCTCCG789680.1173376705438212No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGA789150.1172589184348806No Hit
TGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTC787200.11696917010953305No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA786220.11682355300243531No Hit
CTGACTTTCAATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTACGAAAC765040.11367644042250655No Hit
CCCGACCCAGAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTTCCCCA763080.11338520620831107No Hit
CATTGTTCCAACATGCCAGAGGCTGTTCACCTTGGAGACCTGCTGCGGAT747280.11103750182857196No Hit
CCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCG743630.11049515239907527No Hit
CCCATTCCAGGGCGCCCTGCCCTTCACAAAGAAAAGAGAACTCTCCCCGGG735390.1092707799883759No Hit
GGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCA732100.1087819225574049No Hit
CTCTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCA728200.10820242590670981No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT724710.1076838506987801No Hit
CGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGG718340.10673733950264477No Hit
GTGGTATTTCACCGGCGGCCCGCAGGGCCGGCGGACCCCGCCCCGGGCCC717160.10656200461858831No Hit
CTCGCGGGGACACCGGGGGGGCGCCGGGGGCCTCCCACTTATTCTACAC708510.10527671076512354No Hit
CTGCCCTTCACAAAGAAAAGAGAACTCTCCCCGGGGCTCCCGCCGGCTT708410.10526185187664419No Hit
CTACATTGTTCCAACATGCCAGAGGCTGTTCACCTTGGAGACCTGCTGCGG708380.10525739421010037No Hit
GTTGAGATCGTTTCGGCCCCAAGACCTCTAATCATTCGCTTTACCGGATA706130.10492306921931474No Hit
CACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCA697470.10363628947700206No Hit
GGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGG690460.1025946813945988No Hit
GTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCAC679250.10092899999606239No Hit
CAGCGAGGGAGCTGCTCTGCTACGTACGAAACCCCGACCCAGAAGCAGGT678980.10088888099716811No Hit
CGACACGGCCGGACCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGG678810.1008636208867532No Hit
CTTTTCTGGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTT676550.10052781000711965No Hit
CTCCGACGCACACCACACGCGCGCGCGCGCGCGCCGCCCCCGCCGCTCCCG674870.10027818068066637No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)