FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005204568

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005204568
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35976693
Sequences flagged as poor quality0
Sequence length35-51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTG776920.21595092133676655No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTC642540.17859896127751373No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT632150.17571098043947508No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG521720.14501610806752027No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC478930.1331222967047027No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCT439830.1222541493738738No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGAT431940.12006106286645078No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCA420380.11684787148168399No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC416360.11573048139805402No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC385780.10723053394596328No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG384960.10700260860552135No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC362320.10070964554746596No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT46700.043.31505645
TCGGCGC24200.037.67115445
TGCGCGT29500.029.73696145
GACCTTG222750.027.3359445
AGAACGA54950.026.55097644
TTCGGCG27350.023.84819644
ACCGCGT57100.022.8064046
GACCGCG57400.022.7647085
CCGCGTT58200.022.4868497
CGCGTTC58800.022.2194468
GGACCTT194500.022.16309744
ACGGTAC144700.021.94378745
AGACCGC61300.021.2435464
CAGACCG63750.020.811173
ACTATCG59550.020.16146745
ATACGGA65200.019.94227833
TTGATGC184650.019.52497145
TACGGAG66300.019.51034234
AATACGG68150.019.11165832
GCGTTCT70400.018.7163379
Produced by FastQC (version 0.11.5)