FastQCFastQC Report
Thu 2 May 2024
EGAF00005204570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005204570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54887721
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTT2372730.4322879428715942No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCA2070840.37728657016020034No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA1633650.29763487538496997No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCT1555840.2834586628218723No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGA1296190.2361530004133347No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1254350.22853016615501306No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA1251030.22792529498537573No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA1248140.22739876556361305No Hit
CTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCG1185050.2159043914393895No Hit
CTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTACCCAT1169490.21306951330699264No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG1133310.20647787507883592No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTAC1132850.2063940676276211No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG1128060.20552137699431902No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG1067650.19451527236847746No Hit
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGA1053230.19188809096300427No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGG1050980.19147816321249703No Hit
CTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCT1048050.1909443461862809No Hit
CTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT1012930.18454582947614095No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA1001930.1825417382514388No Hit
CTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGACTTCC983010.1790947013449511No Hit
GCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCG966310.17605212648563054No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT933420.17005989372377112No Hit
CGGGCTTCTTACCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGGC933350.17004714041597757No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCAC926840.16886108279117656No Hit
CAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGG899590.16389640225725532No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT882830.1608428959912546No Hit
CCCTTACCTACATTGTTCCAACATGCCAGAGGCTGTTCACCTTGGAGACC859760.15663977012272018No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTC841440.1533020472830344No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC839990.15303787162159638No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG837700.15262065626663568No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT834960.1521214553615735No Hit
CTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTT834770.15208683924041955No Hit
CTGGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTT830430.15129613415721888No Hit
CTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAGC822790.14990420170660756No Hit
CTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAAT812700.14806590348322168No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC802110.14613651020416754No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT793310.1445332372244058No Hit
CCGGATAAAACTGCGTGGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGC793150.14450408680659196No Hit
CTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGA791170.14414335038614556No Hit
CCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGC776200.14141596441943727No Hit
ACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCT774190.1410497622956508No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC742500.13527615766739523No Hit
CTGCTGTCTATATCAACCAACACCTTTTCTGGGGTCTGATGAGCGTCGGC742450.13526704816182838No Hit
CTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGG726940.13244127953499837No Hit
GTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGT722110.13156130129724278No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC713580.13000721964754192No Hit
CTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTC708480.12907805007972548No Hit
CCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCG708440.12907076247527202No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT708320.12904889966191163No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT697260.1270338770305293No Hit
CTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATT685300.12485488329894404No Hit
CGGATAAAACTGCGTGGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCT673740.1227487656118934No Hit
CGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG672660.12255200029164992No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGG671830.12240078249924058No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA665640.12127302571006728No Hit
CTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTG662290.1206626888370898No Hit
CGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC659110.12008332428303954No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC651580.11871143274467526No Hit
CTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCG651490.11869503563465497No Hit
CCCGGAAGCTGCCCGGCGGGTCATGGGAATAACGCCGCCGCATCGCCGGT645920.11768023671451033No Hit
CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCG640370.11666908159659244No Hit
CTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAAC640310.11665815018991224No Hit
CCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAG639720.11655065802422367No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA634680.11563241986308742No Hit
CTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGA633770.11546662686177113No Hit
CTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA631920.1151295751557985No Hit
CTGCTTACCAAAAGTGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCC629900.11476155113089866No Hit
CCCGCCGCTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCC629480.1146850312841373No Hit
CTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCATCC624630.113801409244155No Hit
CCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGGCCCCAAGACCTC618840.11274652849951632No Hit
CACCTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGG605440.11030518100760642No Hit
GTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGC603700.10998817021388081No Hit
CTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGC603250.10990618466377937No Hit
CACAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGAACCATAACTG598150.1089770150959629No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT594030.10822639183725628No Hit
CTTACCTACATTGTTCCAACATGCCAGAGGCTGTTCACCTTGGAGACCTG593510.10813165297936128No Hit
CGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAG590690.10761787686539216No Hit
GCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCG585310.10663769406640147No Hit
CTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAAC582060.10604557620455766No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA581480.10593990593998247No Hit
CTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTC566410.10319430096214051No Hit
CTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAG560550.10212666690970827No Hit
CGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCC559190.10187888835829055No Hit
CTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAAC556510.10139061885990858No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)