FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005204573

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005204573
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33951810
Sequences flagged as poor quality0
Sequence length35-51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG453980.1337130479936121No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG424800.12511851356378348No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAT41050.029.1274551
CGGTGGC144600.025.8845651
GGCGATC48750.024.4716172
TGGCGCG187150.019.263354
GGTGGCG190400.019.1361372
GGCGCGT188600.019.0341075
GCGCGTG199100.018.0539326
GTGGCGC209200.017.2757663
CGCGTGC231600.015.4539167
CGATCTG81650.014.7723534
GCGTGCC244250.014.7360548
GCGATCT90000.014.0943593
TCCTCGG36300.013.1213842
CGTGCCT279350.013.0438689
GTACCGT23800.012.70794245
CTCCTCG38200.012.5901771
CGTAGGG25000.012.55335837
TGTCGCA51200.011.912642539
GCCACGA40150.011.80594219
ACGAGCT40300.011.65071722
Produced by FastQC (version 0.11.5)