FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005207787

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005207787
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60151550
Sequences flagged as poor quality0
Sequence length25-101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA674900.09.11669294-95
AGATCGG778200.08.82534694-95
ATCGGAA626600.08.77899294-95
TAGATCG256900.08.34989294-95
TCGGAAG639750.07.64993194-95
CGGAAGA668600.07.26591694-95
ACACGTC234300.07.21909694-95
AAGATCG312500.06.90273494-95
CAGATCG299700.06.796568494-95
CACACGT307800.06.715317294-95
GCACACG367950.06.507530794-95
CGTCTGA181750.05.967322394-95
ACGTCTG226700.05.92383294-95
CACGTCT267600.05.849225594-95
GAGATCG343450.05.747097594-95
AGAGCAC679250.05.382792594-95
Produced by FastQC (version 0.11.5)