FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005207793

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005207793
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70814649
Sequences flagged as poor quality0
Sequence length25-101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC445500.09.6979694-95
CGTCTGA320450.09.38637694-95
ATCGGAA1339450.08.96452994-95
AGATCGG1642650.08.72778194-95
GATCGGA1444000.08.69024694-95
TAGATCG506950.08.64627694-95
CACACGT550100.08.31591594-95
ACGTCTG395200.08.29946294-95
TCGGAAG1302000.08.21425594-95
CACGTCT461400.07.989980794-95
CGGAAGA1284150.07.811546394-95
GCACACG655050.07.59063494-95
AAGATCG547450.07.061782494-95
CAGATCG570800.06.91433294-95
AGAGCAC1126000.06.5162394-95
AGCACAC948150.06.42353794-95
GAGATCG624000.06.13797194-95
GAGCACA1112800.05.90837994-95
GAAGAGC1622200.05.8187694-95
AAGAGCA1459250.05.767783694-95
Produced by FastQC (version 0.11.5)