FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005207809

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005207809
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54241089
Sequences flagged as poor quality0
Sequence length25-101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC323300.09.84795294-95
ATCGGAA987100.08.9966694-95
CGTCTGA229000.08.96253194-95
AGATCGG1222150.08.81597294-95
GATCGGA1071000.08.8129794-95
TAGATCG381600.08.54319894-95
ACGTCTG281800.08.46285494-95
CACGTCT338700.08.20265294-95
CACACGT406100.08.12541294-95
TCGGAAG953000.08.06415394-95
CGGAAGA942000.08.04824794-95
GCACACG471900.07.554672294-95
AAGATCG427400.07.335161794-95
CAGATCG414550.07.26091594-95
AGAGCAC828500.06.59992394-95
AGCACAC701450.06.52147294-95
GAGATCG454550.06.286501494-95
GAGCACA832450.06.02274994-95
GAAGAGC1200250.05.80584794-95
AAGAGCA1092200.05.80501694-95
Produced by FastQC (version 0.11.5)