FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005207815

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005207815
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57526046
Sequences flagged as poor quality0
Sequence length25-101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA700450.08.82250894-95
AGATCGG807900.08.66649294-95
ATCGGAA653400.08.61917694-95
TAGATCG258650.08.33285794-95
ACACGTC247950.07.95580394-95
TCGGAAG659300.07.17531794-95
CGGAAGA692300.07.00917994-95
AAGATCG320700.06.749068394-95
CACACGT321150.06.654299394-95
CAGATCG303000.06.51036894-95
GCACACG385900.06.20830694-95
CACGTCT276200.06.205229894-95
ACGTCTG236950.06.14107494-95
CGTCTGA191850.06.140762394-95
AGAGCAC682050.05.4898794-95
GAGATCG363600.05.375072594-95
Produced by FastQC (version 0.11.5)