FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005207819

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005207819
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48771830
Sequences flagged as poor quality0
Sequence length25-101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA657850.08.98602994-95
ACACGTC222600.08.905951594-95
ATCGGAA612700.08.89062694-95
AGATCGG751450.08.74745994-95
TAGATCG239250.08.148478594-95
TCGGAAG609950.07.93404594-95
CGGAAGA618250.07.21237294-95
CGTCTGA171950.07.210172794-95
AAGATCG290950.06.937277394-95
GCACACG343850.06.90639994-95
CACACGT283950.06.855182694-95
CACGTCT249200.06.837726694-95
CAGATCG275450.06.403538794-95
ACGTCTG212050.06.340967794-95
GAGATCG306350.06.187758494-95
AGAGCAC597550.05.5127294-95
AGCACAC524300.05.42043294-95
Produced by FastQC (version 0.11.5)