FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005207825

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005207825
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55694004
Sequences flagged as poor quality0
Sequence length25-101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG804500.08.70349694-95
GATCGGA697600.08.63697994-95
ATCGGAA651800.08.54358194-95
TAGATCG252550.08.35004994-95
ACACGTC250200.07.73522694-95
CGTCTGA186150.07.58504594-95
TCGGAAG659600.07.066543692-93
CGGAAGA696050.06.86813794-95
ACGTCTG240750.06.825439594-95
CACACGT318750.06.654045694-95
GCACACG382350.06.64551494-95
AAGATCG317400.06.64399894-95
CACGTCT278250.06.474253794-95
CAGATCG304450.06.226948394-95
GAGATCG340300.05.374223794-95
AGAGCAC676850.05.28261194-95
Produced by FastQC (version 0.11.5)