FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005207829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005207829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60396185
Sequences flagged as poor quality0
Sequence length25-101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA718250.09.17133894-95
GATCGGA767900.09.12325294-95
TAGATCG285800.08.99358894-95
AGATCGG880050.08.95909694-95
TCGGAAG721950.07.874086494-95
ACACGTC263850.07.80474894-95
CGGAAGA742100.07.362249494-95
CGTCTGA198800.07.29158894-95
CACACGT346050.06.874972394-95
AAGATCG344700.06.73715494-95
CAGATCG333000.06.677677694-95
ACGTCTG250300.06.674945494-95
GCACACG402650.06.487546494-95
CACGTCT297050.06.248255794-95
GAGATCG366600.05.861829394-95
AGAGCAC733650.05.19011994-95
Produced by FastQC (version 0.11.5)