FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005207831

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005207831
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50586772
Sequences flagged as poor quality0
Sequence length25-101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA553200.08.76718994-95
AGATCGG676200.08.52903594-95
GATCGGA586250.08.52597994-95
TAGATCG208000.08.34010194-95
ACACGTC215650.07.76346794-95
TCGGAAG570150.07.110030794-95
ACGTCTG208200.06.776184694-95
CGGAAGA587250.06.624607694-95
CACACGT281550.06.613016694-95
AAGATCG268050.06.47170794-95
CGTCTGA163250.06.37172692-93
CACGTCT240400.06.334517594-95
GCACACG328100.06.228415594-95
CAGATCG260400.06.01076394-95
GAGATCG305200.05.316921794-95
Produced by FastQC (version 0.11.5)