FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005207847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005207847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52199722
Sequences flagged as poor quality0
Sequence length25-101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCG287550.09.41162494-95
GATCGGA799400.09.359488594-95
ATCGGAA733050.08.99862594-95
AGATCGG908950.08.99374794-95
ACACGTC257650.08.98433594-95
CGTCTGA194950.07.77779394-95
CACACGT334200.07.688804694-95
TCGGAAG721400.07.339276394-95
CGGAAGA737750.07.21031694-95
CAGATCG336650.07.088304594-95
ACGTCTG241850.07.02750294-95
GCACACG398950.06.95493194-95
AAGATCG330750.06.86716294-95
CACGTCT280450.06.71393294-95
AGAGCAC696750.05.971224394-95
GAGATCG372950.05.923505394-95
AGCACAC595000.05.68683694-95
GAGCACA711350.05.337620394-95
GAAGAGC1023900.05.12544994-95
AAGAGCA930900.05.07675294-95
Produced by FastQC (version 0.11.5)