FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005207865

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005207865
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56501886
Sequences flagged as poor quality0
Sequence length25-101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA832100.09.55816494-95
GATCGGA903500.09.42777394-95
TAGATCG315600.09.13693694-95
AGATCGG1042700.08.84390194-95
ACACGTC282750.08.72476394-95
TCGGAAG826550.08.21588994-95
CGTCTGA206900.08.12884694-95
CGGAAGA844450.07.82719394-95
CACACGT365500.07.50123494-95
ACGTCTG264100.07.237182694-95
AAGATCG372500.07.14140894-95
GCACACG453450.07.125060694-95
CACGTCT309200.06.64054494-95
CAGATCG402650.06.636663494-95
AGAGCAC771450.06.1646394-95
GAGATCG438600.06.0513894-95
AGCACAC667500.05.916851594-95
GAGCACA788750.05.485813694-95
AAGAGCA1016000.05.373273494-95
GAAGAGC1140100.05.16976894-95
Produced by FastQC (version 0.11.5)