FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005207873

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005207873
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55694621
Sequences flagged as poor quality0
Sequence length25-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA908450.09.93538294-95
ATCGGAA833750.09.80371894-95
AGATCGG1050500.09.42598794-95
TAGATCG318950.09.2870794-95
ACACGTC279950.08.93470694-95
TCGGAAG821700.08.06203994-95
CGGAAGA835250.07.81146694-95
CACACGT366300.07.507185594-95
CAGATCG387850.07.23077394-95
CGTCTGA203400.07.11007594-95
AAGATCG375800.07.002767694-95
CACGTCT307900.06.98141394-95
GCACACG438900.06.85946894-95
ACGTCTG265300.06.845360894-95
AGAGCAC764500.06.42457394-95
AGCACAC647000.05.857502594-95
GAGATCG448300.05.79587394-95
GAGCACA788350.05.43028294-95
GAAGAGC1127000.05.36423894-95
AAGAGCA999250.05.294537594-95
Produced by FastQC (version 0.11.5)