FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005207875

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005207875
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46821750
Sequences flagged as poor quality0
Sequence length25-101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA795300.09.38439994-95
GATCGGA866550.09.32880194-95
TAGATCG292850.08.99426594-95
AGATCGG997800.08.98959494-95
ACACGTC277650.08.65939494-95
CGTCTGA198200.08.59374894-95
TCGGAAG787550.08.04879794-95
CGGAAGA798600.07.81042994-95
CACACGT349100.07.79282994-95
ACGTCTG252900.07.63142594-95
GCACACG424100.07.35721794-95
AAGATCG340450.07.27235294-95
CAGATCG365900.07.08447294-95
CACGTCT294000.06.909552694-95
AGAGCAC703900.06.2253194-95
GAGATCG421700.06.12582394-95
AGCACAC600100.05.97987694-95
AAGAGCA894900.05.599960394-95
GAGCACA716750.05.52272494-95
GAAGAGC1039200.05.450997494-95
Produced by FastQC (version 0.11.5)