FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005207879

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005207879
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47968604
Sequences flagged as poor quality0
Sequence length25-101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA713150.012.15997694-95
ACGTCTG842350.011.52307494-95
ACACGTC1059400.010.88043194-95
CACGTCT983600.010.61499994-95
CACACGT1234650.010.10876194-95
TCTGAAC695450.09.77838794-95
GTCTGAA785800.09.48626894-95
GCACACG1452250.09.17880294-95
CTGAACT695950.09.14027294-95
AGCACAC1706350.08.79147894-95
AGAGCAC2092250.08.5589394-95
GAGCACA1958950.08.49363394-95
ATCGGAA3305600.08.33595294-95
TGAACTC684350.08.25051994-95
CGGAAGA2912650.08.15537494-95
TCGGAAG3085950.08.11566294-95
GATCGGA3604300.08.05266794-95
AAGAGCA2420200.07.94055194-95
AGATCGG3982000.07.82380194-95
AACTCCA581500.07.808953394-95
Produced by FastQC (version 0.11.5)