FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005207895

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005207895
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61374653
Sequences flagged as poor quality0
Sequence length25-101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC546150.010.85543994-95
CGTCTGA369750.010.44080894-95
ACGTCTG452600.09.74329994-95
CACACGT664250.09.59614194-95
ATCGGAA1793150.09.54056494-95
AGATCGG2258350.09.31050294-95
CACGTCT541900.09.30556294-95
GATCGGA1971400.09.26090494-95
TAGATCG641900.09.21250894-95
GCACACG827650.08.67730894-95
TCGGAAG1701850.08.63035994-95
CGGAAGA1668500.08.62534994-95
AGAGCAC1291050.08.14116994-95
AAGATCG716650.08.02906194-95
AGCACAC1046700.07.966614294-95
CAGATCG743650.07.747759394-95
GAGATCG846800.07.45868394-95
GAGCACA1241550.07.36063794-95
AAGAGCA1583400.07.24235894-95
GAAGAGC1829350.07.03804494-95
Produced by FastQC (version 0.11.5)