FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005207896

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005207896
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61374653
Sequences flagged as poor quality0
Sequence length25-101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG137350.018.10900794-95
GATCGGA215200.014.39886888-89
ATCGGAA225350.014.24502588-89
AGATCGG255200.012.83724386-87
GAGCGTC215500.011.66731792-93
AGAGCGT213700.011.61361392-93
TAGATCG74000.010.69885986-87
AAGAGCG340150.010.04542494-95
TCGGAAG342550.09.47712390-91
CGGAAGA464000.07.587070590-91
CAGATCG194750.05.08480186-87
Produced by FastQC (version 0.11.5)