FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005207907

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005207907
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51344579
Sequences flagged as poor quality0
Sequence length25-101
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1069000.09.43405894-95
GATCGGA1170800.09.34708294-95
TAGATCG388300.09.2101794-95
ACACGTC365050.08.9355194-95
AGATCGG1335550.08.92970594-95
CGTCTGA254450.08.79183494-95
TCGGAAG1049200.08.67278394-95
CACACGT446850.08.27398994-95
ACGTCTG318050.08.20286694-95
CGGAAGA1035750.07.889352394-95
CACGTCT377800.07.84977794-95
GCACACG562450.07.449541694-95
CAGATCG479250.07.43707194-95
AAGATCG444950.07.03200894-95
AGAGCAC871200.06.66591494-95
AGCACAC735300.06.532953794-95
GAGATCG516900.06.485975394-95
GAGCACA874400.06.154036594-95
AAGAGCA1081400.06.052318694-95
TTAGATC205050.05.897262694-95
Produced by FastQC (version 0.11.5)