FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005207913

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005207913
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54464291
Sequences flagged as poor quality0
Sequence length25-101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC429350.010.02118794-95
CGTCTGA300600.010.01282494-95
ACGTCTG372250.09.70005394-95
CACGTCT433350.09.13054394-95
GATCGGA1503150.08.92170394-95
ATCGGAA1382050.08.89586794-95
CACACGT521450.08.82513194-95
TAGATCG538350.08.67111894-95
AGATCGG1683050.08.62685294-95
CGGAAGA1273250.08.55402194-95
GCACACG599250.08.50216494-95
TCGGAAG1304400.08.25007494-95
AGAGCAC1016400.07.63853594-95
AAGATCG571150.07.284113494-95
AGCACAC834400.07.24675694-95
CAGATCG508450.07.014830694-95
GAGCACA989100.06.90446594-95
GAGATCG592250.06.730530794-95
GAAGAGC1445450.06.504842894-95
AAGAGCA1318800.06.47107694-95
Produced by FastQC (version 0.11.5)