FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005207915

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005207915
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58921995
Sequences flagged as poor quality0
Sequence length25-101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA379800.011.24349694-95
ACACGTC553100.010.72507694-95
ACGTCTG461000.010.30063194-95
CACGTCT542950.09.98559294-95
CACACGT670100.09.904757594-95
ATCGGAA1897300.09.22506894-95
GCACACG770200.09.21608794-95
CGGAAGA1690400.09.04296194-95
GATCGGA2063750.08.96128994-95
AGATCGG2333000.08.85600294-95
TAGATCG733350.08.78825694-95
AGAGCAC1296700.08.21776494-95
TCGGAAG1739500.08.20233494-95
AGCACAC1067500.07.804206494-95
AAGATCG734100.07.6776794-95
GAGCACA1235850.07.632865494-95
GAGATCG816050.07.538105594-95
CAGATCG673500.07.530175794-95
GAAGAGC1810600.07.176233394-95
AAGAGCA1647250.06.83864594-95
Produced by FastQC (version 0.11.5)