FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005207919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005207919
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55150303
Sequences flagged as poor quality0
Sequence length25-101
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA314850.09.85765194-95
ACACGTC460000.09.78225194-95
ATCGGAA1442000.09.50793194-95
AGATCGG1826600.09.3989894-95
ACGTCTG389800.09.29572294-95
GATCGGA1586150.09.20902194-95
CACACGT560450.09.05499294-95
TAGATCG514600.09.03223694-95
CACGTCT460450.08.46930294-95
CGGAAGA1368800.08.46256594-95
TCGGAAG1381100.08.33992794-95
AAGATCG570600.07.93455594-95
GCACACG686650.07.80766394-95
CAGATCG621300.07.74377694-95
AGAGCAC1073100.07.635465694-95
AGCACAC882750.07.489826794-95
GAGATCG720050.07.208359794-95
GAGCACA1043800.06.87308394-95
GAAGAGC1541800.06.82083194-95
AAGAGCA1308800.06.80528894-95
Produced by FastQC (version 0.11.5)