FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005207925

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005207925
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56605135
Sequences flagged as poor quality0
Sequence length25-101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA701150.010.62658694-95
ATCGGAA644550.010.25760794-95
TAGATCG271450.010.13377294-95
AGATCGG794750.09.87506594-95
TCGGAAG629100.08.61454294-95
AAGATCG326950.08.15161594-95
CAGATCG312000.07.905390394-95
ACACGTC219000.07.85244794-95
CGTCTGA172350.07.67222794-95
CACACGT288900.07.363587494-95
GCACACG346300.07.13227794-95
CGGAAGA639550.07.065057894-95
GAGATCG302850.06.87735494-95
CACGTCT249100.06.17473194-95
ACGTCTG211900.06.127081494-95
AGAGCAC632900.05.363940794-95
Produced by FastQC (version 0.11.5)