FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005207933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005207933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50653883
Sequences flagged as poor quality0
Sequence length25-101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA497600.08.93754994-95
AGATCGG608300.08.82864994-95
GATCGGA526950.08.82207494-95
TAGATCG200750.08.552855594-95
TCGGAAG513050.07.67789694-95
ACACGTC194250.07.41489694-95
CGTCTGA154100.06.888151694-95
AAGATCG254000.06.77162594-95
CGGAAGA539600.06.66484294-95
CAGATCG237900.06.572634794-95
ACGTCTG195150.05.94770494-95
CACACGT257100.05.84788194-95
CACGTCT225100.05.663970594-95
GCACACG307850.05.49920494-95
GAGATCG280100.05.217395394-95
Produced by FastQC (version 0.11.5)