FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005207935

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005207935
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53006808
Sequences flagged as poor quality0
Sequence length25-101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA554150.010.80079294-95
AGATCGG641000.010.20039594-95
TAGATCG219450.010.11830494-95
ATCGGAA504550.010.09612694-95
AAGATCG271300.08.12788694-95
CAGATCG256200.07.78222494-95
TCGGAAG506150.07.696161394-95
ACACGTC192200.07.695251594-95
CGTCTGA157100.07.360473694-95
CGGAAGA528750.06.778704694-95
CACACGT253050.06.619049594-95
GAGATCG272800.06.210259494-95
ACGTCTG192450.06.088319394-95
CACGTCT218550.06.064343594-95
GCACACG299200.05.829210894-95
AGAGCAC552900.05.051301594-95
Produced by FastQC (version 0.11.5)