FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005207937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005207937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55427543
Sequences flagged as poor quality0
Sequence length25-101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA807600.010.59016594-95
TAGATCG304250.010.20315494-95
ATCGGAA735700.010.1249794-95
AGATCGG927650.010.00527794-95
CGTCTGA189000.08.91691494-95
AAGATCG352550.08.4773494-95
ACACGTC240700.08.44273694-95
TCGGAAG713650.08.26285694-95
CAGATCG341050.07.67421394-95
CACACGT325550.07.619857394-95
CGGAAGA717900.07.24438892-93
ACGTCTG236500.07.2148894-95
CACGTCT270300.07.10339294-95
GAGATCG354600.07.035136794-95
GCACACG378400.06.907469794-95
AGAGCAC673100.05.77275794-95
GAAGAGC1021050.05.493835494-95
AGCACAC582950.05.457400394-95
Produced by FastQC (version 0.11.5)