FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005207943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005207943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54492423
Sequences flagged as poor quality0
Sequence length25-101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA587450.09.18120594-95
AGATCGG679650.08.84846794-95
ATCGGAA555850.08.67900794-95
TAGATCG219000.08.54254294-95
TCGGAAG571100.07.82531694-95
ACACGTC208550.07.66408194-95
CGGAAGA591300.07.278214594-95
CACACGT278500.06.73494694-95
AAGATCG278500.06.43794494-95
GCACACG326900.06.31082194-95
CGTCTGA167050.06.26219994-95
CAGATCG272350.06.16348994-95
ACGTCTG208450.05.602014594-95
CACGTCT239750.05.509930694-95
GAGATCG308000.05.221023694-95
AGAGCAC603200.05.114024694-95
Produced by FastQC (version 0.11.5)