FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005207945

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005207945
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55119348
Sequences flagged as poor quality0
Sequence length25-101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA704300.011.22846694-95
TAGATCG263050.010.98651694-95
AGATCGG808500.010.28485994-95
ATCGGAA634500.09.71938794-95
AAGATCG326000.09.099956594-95
CGTCTGA177700.08.50595394-95
CAGATCG314350.08.23191194-95
ACACGTC224750.08.1958894-95
TCGGAAG642100.07.959681594-95
GAGATCG343850.07.443613594-95
GCACACG364850.07.412884794-95
ACGTCTG213300.07.21857894-95
CGGAAGA652400.07.097719792-93
CACACGT291650.06.19638592-93
CACGTCT254500.06.17669294-95
AGAGCAC648750.05.54200194-95
AGCACAC569200.05.240176794-95
TTACGCG12000.0096380725.05180261
Produced by FastQC (version 0.11.5)