FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005207951

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005207951
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47191517
Sequences flagged as poor quality0
Sequence length25-101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCG205950.09.3641994-95
GATCGGA530900.09.06295594-95
AGATCGG609600.08.90431294-95
ATCGGAA502100.08.56606994-95
ACACGTC181600.07.930896894-95
TCGGAAG505900.07.380511894-95
CGGAAGA531050.06.92416794-95
CGTCTGA146800.06.753443794-95
CACACGT247650.06.66212994-95
AAGATCG246600.06.44047894-95
CACGTCT214350.06.224422594-95
GCACACG288250.06.09166494-95
ACGTCTG185400.05.932673594-95
CAGATCG243950.05.82300294-95
AGAGCAC530700.05.33015294-95
GAGATCG259700.05.071011594-95
Produced by FastQC (version 0.11.5)