FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005207973

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005207973
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55534304
Sequences flagged as poor quality0
Sequence length25-101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA542550.09.3045194-95
GATCGGA575650.09.02697194-95
AGATCGG667400.08.71427894-95
TAGATCG223450.08.22488794-95
TCGGAAG560550.07.64138694-95
CGGAAGA580850.06.97626894-95
ACACGTC208900.06.882115494-95
AAGATCG271650.06.72186394-95
CACACGT276250.06.470437594-95
CGTCTGA168550.06.13783694-95
GCACACG331050.05.99033694-95
ACGTCTG206800.05.76227794-95
CAGATCG269750.05.604377394-95
CACGTCT240500.05.349254694-95
GAGATCG307100.05.08685694-95
Produced by FastQC (version 0.11.5)