FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005207981

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005207981
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56720463
Sequences flagged as poor quality0
Sequence length25-101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA435800.013.3461694-95
GATCGGA2262800.010.8770194-95
CACACGT763150.010.83121594-95
TAGATCG722850.010.72281594-95
ACACGTC613650.010.68420394-95
ACGTCTG527200.010.29991494-95
ATCGGAA1980450.09.83413494-95
CACGTCT622700.09.7473394-95
AAGATCG730950.09.64099194-95
AGATCGG2519900.09.61401694-95
CAGATCG871950.09.0645594-95
GCACACG930100.08.70541394-95
GTCTGAA538650.08.67710294-95
AGCACAC1135400.08.6192394-95
GAGATCG919800.08.57987394-95
AGAGCAC1383650.08.43328494-95
TCGGAAG1860100.08.38584494-95
TCTGAAC503150.08.09018294-95
CGGAAGA1781350.07.8319392-93
GAAGAGC1919250.07.32471894-95
Produced by FastQC (version 0.11.5)