FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005207983

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005207983
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58820238
Sequences flagged as poor quality0
Sequence length25-101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC366150.09.46613894-95
GATCGGA1258050.09.05016994-95
ATCGGAA1154050.09.0058294-95
CGTCTGA268300.08.99990194-95
TAGATCG427650.08.82856894-95
AGATCGG1421800.08.7663194-95
CACACGT459550.08.54713594-95
ACGTCTG326250.08.18434494-95
GCACACG550350.07.985024594-95
CGGAAGA1107450.07.967408794-95
TCGGAAG1112000.07.91934594-95
CACGTCT381450.07.87578394-95
AAGATCG493300.07.17061394-95
AGAGCAC944050.06.817280394-95
CAGATCG493150.06.78924894-95
AGCACAC797800.06.72147594-95
GAGATCG522700.06.461823594-95
GAGCACA928200.06.18474894-95
GAAGAGC1359200.06.083249694-95
AAGAGCA1220300.05.946326794-95
Produced by FastQC (version 0.11.5)