FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005207985

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005207985
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58978333
Sequences flagged as poor quality0
Sequence length25-101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA420750.011.30569794-95
ACACGTC596700.010.28802394-95
ACGTCTG512550.09.99592794-95
CACGTCT602800.09.41820694-95
CACACGT730150.09.36334594-95
GATCGGA2014550.09.28734694-95
ATCGGAA1824850.09.14137394-95
TAGATCG648850.08.96318994-95
AGATCGG2260650.08.87921994-95
GCACACG883550.08.46906694-95
AAGATCG704600.08.09985994-95
CAGATCG759150.07.821833694-95
CGGAAGA1677550.07.681387494-95
AGAGCAC1320950.07.654392294-95
TCGGAAG1704650.07.637082692-93
AGCACAC1102200.07.532939494-95
GAGATCG799550.07.345109594-95
GTCTGAA589250.07.04523294-95
TCTGAAC555450.06.906923394-95
GAAGAGC1859650.06.80365294-95
Produced by FastQC (version 0.11.5)