FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005207989

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005207989
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51584590
Sequences flagged as poor quality0
Sequence length25-101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC369550.08.92087794-95
ATCGGAA1027350.08.87816394-95
GATCGGA1122900.08.71296294-95
CGTCTGA266100.08.67136494-95
AGATCGG1284200.08.60075794-95
TAGATCG366450.08.17297294-95
ACGTCTG339300.08.13450194-95
TCGGAAG1019850.07.88372294-95
CACACGT462100.07.80854294-95
CACGTCT398500.07.776306694-95
CGGAAGA1031450.07.43539894-95
GCACACG572650.06.944602594-95
AAGATCG415500.06.77363694-95
CAGATCG466300.06.650935694-95
AGAGCAC893200.06.440381594-95
GAGATCG529450.06.390160694-95
AGCACAC760950.06.298661794-95
GAGCACA887600.05.88752694-95
AAGAGCA1062750.05.71541694-95
GAAGAGC1271300.05.567653794-95
Produced by FastQC (version 0.11.5)